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Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8468] to 67kDa Laminin Receptor
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-67kDa Laminin Receptor antibody [EPR8468]
    See all 67kDa Laminin Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR8468] to 67kDa Laminin Receptor

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    A synthetic peptide corresponding to residues in Human 67kDa Laminin Receptor (UniProt ID: P08865)

  • Positive control

    • WB: Lysates of K562, HeLa, HepG2, C6, RAW 264.7, PC 12, NIH/3T3 IHC-P: Human brain tissue, Human kidney tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
    Western blot - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
    All lanes : Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775) at 1/2000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line)
    Lane 4 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa
    Observed band size: 43 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 60 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)

    ab133775, at 1/250, staining 67kDa Laminin Receptor in paraffin-embedded Human brain tissue using immunohistochemical analysis.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)

    ab133775, at 1/250, staining 67kDa Laminin Receptor in paraffin-embedded Human kidney tissue using immunohistochemical analysis.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
    OI-RD Scanning - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)
    Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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