Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Wednesday March 10, 2021

Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8468] to 67kDa Laminin Receptor
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-67kDa Laminin Receptor antibody [EPR8468]
See all 67kDa Laminin Receptor primary antibodies
Description

Rabbit monoclonal [EPR8468] to 67kDa Laminin Receptor
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

A synthetic peptide corresponding to residues in Human 67kDa Laminin Receptor (UniProt ID: P08865)
Positive control
- WB: Lysates of K562, HeLa, HepG2, C6, RAW 264.7, PC 12, NIH/3T3 IHC-P: Human brain tissue, Human kidney tissue
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 6.70 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8468
Isotype

IgG
Research areas

Western blot - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)

All lanes : Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775) at 1/2000 dilution

Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line)
Lane 4 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 43 kDa
why is the actual band size different from the predicted?

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 60 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)

ab133775, at 1/250, staining 67kDa Laminin Receptor in paraffin-embedded Human brain tissue using immunohistochemical analysis.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)

ab133775, at 1/250, staining 67kDa Laminin Receptor in paraffin-embedded Human kidney tissue using immunohistochemical analysis.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
OI-RD Scanning - Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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