Anti-67kDa Laminin Receptor antibody [RPSA/2699] (ab268079)
Key features and details
- Mouse monoclonal [RPSA/2699] to 67kDa Laminin Receptor
- Suitable for: Flow Cyt, Protein Array, IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-67kDa Laminin Receptor antibody [RPSA/2699]
See all 67kDa Laminin Receptor primary antibodies -
Description
Mouse monoclonal [RPSA/2699] to 67kDa Laminin Receptor -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human -
Immunogen
Recombinant full length protein within Human 67kDa Laminin Receptor aa 1-295. The exact sequence is proprietary.
Database link: P08865 -
Positive control
- WB: THP-1, U937, SK-BR-3, and A431 cell lysates. ICC/IF: HeLa and Raji cells. IHC-P: Human breast carcinoma, colon carcinoma, cervix carcinoma, tonsil and basal cell carcinoma tissues. Flow Cyt: Raji cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor concentrate -
Clonality
Monoclonal -
Clone number
RPSA/2699 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Formalin-fixed, paraffin-embedded human basal cell carcinoma tissue stained for 67kDa Laminin Receptor using ab268079 at 2 μg/ml in immunohistochemical analysis.
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Protein Array containing more than 19,000 full-length human proteins using ab268079. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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Immunofluorescent staining of HeLa cells stained for 67kDa Laminin Receptor using ab268079 at 2 µg/ml followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).
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All lanes : Anti-67kDa Laminin Receptor antibody [RPSA/2699] (ab268079) at 2 µg/ml
Lane 1 : THP-1 (human monocytic leukemia cell line) cell lysate
Lane 2 : U937 (human histiocytic lymphoma cell line) cell lysate
Lane 3 : SK-BR-3 (human mammary gland adenocarcinoma cell line) cell lysate
Lane 4 : A431 (human epidermoid carcinoma cell line) cell lysate
Predicted band size: 33 kDa
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Flow Cytometric analysis of paraformaldehyde-fixed Raji cells labelling 67kDa Laminin Receptor using ab268079 at at 2 μg/ml followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).
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Immunofluorescent staining of paraformaldehyde-Raji cells stained for 67kDa Laminin Receptor using ab268079 at 2 µg/ml followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for 67kDa Laminin Receptor using ab268079 at 2 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human cervix carcinoma tissue stained for 67kDa Laminin Receptor using ab268079 at 2 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for 67kDa Laminin Receptor using ab268079 at 2 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for 67kDa Laminin Receptor using ab268079 at 2 μg/ml in immunohistochemical analysis.