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Signal Transduction Cytoskeleton / ECM Extracellular Matrix

Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653)

Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8468] to 67kDa Laminin Receptor - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

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Anti-67kDa Laminin Receptor antibody [EPR8469] (ab133645)

Overview

  • Product name

    Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free
    See all 67kDa Laminin Receptor primary antibodies
  • Description

    Rabbit monoclonal [EPR8468] to 67kDa Laminin Receptor - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248653 is the carrier-free version of ab133775. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248653 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 6.70 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8468
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Extracellular Matrix
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Prions
    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Signal Transduction
    • Cytoskeleton / ECM
    • Basal Lamina
    • Neuroscience
    • Cell Adhesion Proteins
    • ECM Proteins

Images

  • Western blot - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653)
    Western blot - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653)
    All lanes : Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775) at 1/2000 dilution

    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line)
    Lane 4 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa
    Observed band size: 43 kDa
    why is the actual band size different from the predicted?


    Exposure time: 60 seconds


    This data was developed using ab133775, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653)
    This data was developed using ab133775, the same antibody clone in a different buffer formulation.ab133775, at 1/250, staining 67kDa Laminin Receptor in paraffin-embedded Human brain tissue using immunohistochemical analysis. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653)
    This data was developed using ab133775, the same antibody clone in a different buffer formulation.ab133775, at 1/250, staining 67kDa Laminin Receptor in paraffin-embedded Human kidney tissue using immunohistochemical analysis. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653)
    OI-RD Scanning - Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653)
    This data was developed using ab133775, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653)
    Anti-67kDa Laminin Receptor antibody [EPR8468] - BSA and Azide free (ab248653)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: Flow Cyt, ICC/IF, IHC-P, Protein Array, WB

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