Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12713] to OGT / O-Linked N-Acetylglucosamine Transferase
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713]
    See all OGT / O-Linked N-Acetylglucosamine Transferase primary antibodies

  • Description

    Rabbit monoclonal [EPR12713] to OGT / O-Linked N-Acetylglucosamine Transferase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human OGT/ O-Linked N-Acetylglucosamine Transferase aa 1000 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O15294

  • Positive control

    • WB: A549, HeLa, U87-MG and HEK-293T cell lysates. IHC-P: Human pancreas tissue. Flow Cyt: HEK-293T cells. ICC/IF: K562 and A549 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
    Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
    All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941) at 1/1000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : U87-MG cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 116 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
    Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

    ab177941 staining OGT / O-Linked N-Acetylglucosamine Transferase antibody in K562 (human chronic myelogenous leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilised with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1: PBS only.

  • Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
    Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

    Immunofluorescent analysis of A549 cells labeling OGT / O-Linked N-Acetylglucosamine Transferase with ab177941 at 1/50 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling OGT / O-Linked N-Acetylglucosamine Transferase with ab177941 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
    Flow Cytometry - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

    Flow cytometric analysis of permeabilized 293T cells labeling OGT / O-Linked N-Acetylglucosamine Transferase with ab177941 at 1/10 dilution (red)  or a rabbit IgG (negative) (green).

  • Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
    Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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