Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab59135)
Key features and details
- Goat polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody
See all OGT / O-Linked N-Acetylglucosamine Transferase primary antibodies -
Description
Goat polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase -
Host species
Goat -
Specificity
ab59135 is expected to recognize both reported isoforms of O-Linked N-Acetylglucosamine -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Synthetic peptide:
C-YEHPKDLKLSDGR
by a Cysteine residue linker, corresponding to amino acids 543-555 of Human O-Linked N-Acetylglucosamine
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab59135)Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab59135) at 0.05 µg/ml + Rat pancreas lysate in RIPA buffer at 35 µg
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Preliminary testing was unsuccessful on Mouse Brain for this particular batch.
