Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab106319)
Key features and details
- Rabbit polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody
See all OGT / O-Linked N-Acetylglucosamine Transferase primary antibodies -
Description
Rabbit polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Gorilla, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide corresponding to Human OGT/ O-Linked N-Acetylglucosamine Transferase aa 500-600 conjugated to keyhole limpet haemocyanin.
(Peptide available asab129221) -
Positive control
- This antibody gave a positive signal in both HeLa and A549 whole cell lysates and HepG2 cell line.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab106319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa). ICC/IF Use a concentration of 5 µg/ml. Target
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Function
Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. -
Tissue specificity
Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the O-GlcNAc transferase family.
Contains 13 TPR repeats. -
Domain
The TPR repeat domain mediates recognition of protein substrates. -
Post-translational
modificationsUbiquitinated, leading to its proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. Mostly in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 532053 Cow
- Entrez Gene: 8473 Human
- Entrez Gene: 108155 Mouse
- Entrez Gene: 664652 Pig
- Entrez Gene: 100354727 Rabbit
- Entrez Gene: 26295 Rat
- Omim: 300255 Human
- SwissProt: O15294 Human
see all -
Alternative names
- FLJ23071 antibody
- GlcNAc transferase antibody
- HRNT1 antibody
see all
Images
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Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab106319)All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab106319) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 116 kDa
Observed band size: 116 kDa
Additional bands at: 170 kDa, 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab106319)ICC/IF image of ab106319 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106319, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) MCF7 cells at 5µg/ml.
Protocols
Datasheets and documents
References (0)
ab106319 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab106319)All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab106319) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 116 kDa
Observed band size: 116 kDa
Additional bands at: 170 kDa, 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab106319)ICC/IF image of ab106319 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106319, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) MCF7 cells at 5µg/ml.
