Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

Predicted band size: 117 kDa



7.5% SDS PAGE

All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1 µg/ml

Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish heart homogenate at 20 µg
Lane 4 : Zebrafish liver homogenate at 20 µg
Lane 5 : Zebrafish skeletal muscle homogenate at 20 µg
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 117 kDa
Observed band size: 117 kDa


Exposure time: 5 minutes

HeLa cells stained for O-GlcNAc (green) using ab96718 at 1/200 dilution in ICC/IF. 

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using ab96718 at 1/100 dilution.

Immunoprecipitation analysis of OGT/O-linked N-Acetylglucosamine Transferase protein from A431 whole cell extracts using ab96718 (5µg). Western blot analysis was performed using ab96718 and an Easyblot anti-rabbit IgG was used as the secondary antibody. 

Ab96718 staining OGT / O-Linked N-Acetylglucosamine Transferase in MCF7 cells by ICC/IF  (Immunocytochemistry/Immunofluorescence). MFC7 cells were fixed with paraformaldehyde at room temperature for 15 minutes. Samples were incubated with primary antibody at a 1:500 dilution.

Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/1000 dilution + PC-12 whole cell extract at 30 µg

Predicted band size: 117 kDa

5% SDS-PAGE

ICC/IF image of ab96718 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96718, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Paraffin embedded rat hind brain tissue stained for O-GlcNAc using ab96718 at 1/500 in immunohistochemical analysis.

Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/2000 dilution + Mouse brain tissue extract at 50 µg

Predicted band size: 117 kDa

7.5% SDS-PAGE