Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (ab248660)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5088(2)] to RanBP3 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free
    See all RanBP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR5088(2)] to RanBP3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248660 is the carrier-free version of ab134052 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248660 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (ab248660)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (ab248660)
    This data was developed using ab134052, the same antibody clone in a different buffer formulation.Immunohistochemistry of Human clear cell carcinoma of kidney tissues staining RanBP3 using ab134052 (1/1000). a HRP conjugated Goat Anti-Rabbit IgG H&L was used as a secondary antibody. Antigen retrieval performed using heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). image shows nuclear and cytoplasmic staining on human clear cell carcinoma of kidney.
  • Western blot - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (ab248660)
    Western blot - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (ab248660)
    All lanes : Anti-RanBP3 antibody [EPR5088(2)] (ab134052) at 1/1000 dilution

    Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : MEF (Mouse embryonic fibroblast) whole cell lysate
    Lane 3 : Y79 (Human retinoblastoma retinoblastoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution

    Predicted band size: 60 kDa
    Observed band size: 70,90 kDa
    why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    This data was developed using ab134052, the same antibody clone in a different buffer formulation.

    The double bands are consistent with what has been described in PMID 16314428.

    We are unsure how to define the extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (ab248660)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (ab248660)
    This data was developed using ab134052, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling RanBP3 with ab134052 at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (ab248660)
    Anti-RanBP3 antibody [EPR5088(2)] - BSA and Azide free (ab248660)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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