Mouse IgG3 ELISA Kit (ab157721)
Key features and details
- Sensitivity: 1.3189 ng/ml
- Range: 12.5 ng/ml - 2450 ng/ml
- Sample type: Other biological fluids, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
-
Product name
Mouse IgG3 ELISA Kit
See all IgG3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Other biological fluids -
Assay type
Sandwich (quantitative) -
Sensitivity
1.3189 ng/ml -
Range
12.5 ng/ml - 2450 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s IgG3 Mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgG3 in mouse samples.
In this assay the IgG3 present in samples reacts with the anti-IgG3 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG3 antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgG3. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG3 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG3 in the test sample. The quantity of IgG3 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
-
Platform
Microplate
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-conjugated anti-mouse IgG3 antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Mouse IgG3 Calibrator 1 vial Mouse IgG3 ELISA Microplate 1 unit Stop Solution 1 x 12ml -
Research areas
-
Relevance
IgG3, like IgG1, activates complement and has an affinity for fc receptors; thus IgG1 and IgG3 are the more effective of the IgG subclasses. An IgG3 deficiency is associated with Wiscott-Aldrich disease, systemic lupus erythematosus, juvenile diabetes mellitus, and peridontal infections. IgG anti-viral antibodies are restricted to IgG subclasses 1 and 3, with IgG3 antibodies being the first to appear in response to infection. -
Alternative names
- DKFZp686H11213
- FLJ39988
- FLJ40036
see all -
Database links
- Entrez Gene: 380795 Mouse
- SwissProt: P03987 Mouse
- Unigene: 342177 Mouse
Images
-
Standard CurveRepresentative standard curve using ab157721 IgG3 Mouse ELISA Kit