Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ferritin Heavy Chain antibody (ab65080)

Key features and details

  • Rabbit polyclonal to Ferritin Heavy Chain
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Ferritin Heavy Chain antibody
    See all Ferritin Heavy Chain primary antibodies

  • Description

    Rabbit polyclonal to Ferritin Heavy Chain

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Sheep, Goat, Cow, Cat, Dog, Orangutan

  • Immunogen

    Synthetic peptide corresponding to Human Ferritin Heavy Chain aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
    Database link: P02794
    (Peptide available as ab90631)

  • Positive control

    • Recombinant Human Ferritin Heavy Chain protein (ab78877) can be used as a positive control in WB. This antibody gave a positive signal in Human Liver Tissue Lysate. IF/ICC: MCF7 cell line. IHC-P: FFPE human normal liver tissue sections.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab65080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

Images

  • Western blot - Anti-Ferritin Heavy Chain antibody (ab65080)
    Western blot - Anti-Ferritin Heavy Chain antibody (ab65080)
    Anti-Ferritin Heavy Chain antibody (ab65080) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa
    Observed band size: 21 kDa
    Additional bands at: 24 kDa (possible post-translational modification)


    Exposure time: 3 minutes


    Ferritin Heavy Chain contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a slightly higher than predicted molecular weight (24 kDa).
  • Immunocytochemistry/ Immunofluorescence - Anti-Ferritin Heavy Chain antibody (ab65080)
    Immunocytochemistry/ Immunofluorescence - Anti-Ferritin Heavy Chain antibody (ab65080)
    ICC/IF image of ab65080 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65080, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody (ab65080)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody (ab65080)
    IHC image of ab65080 staining in Human Normal Liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65080, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Western blot - Anti-Ferritin Heavy Chain antibody (ab65080)
    Western blot - Anti-Ferritin Heavy Chain antibody (ab65080)
    All lanes : Anti-Ferritin Heavy Chain antibody (ab65080) at 1 µg/ml

    Lane 1 : Recombinant Human Ferritin Heavy Chain protein (ab78877) at 0.1 µg
    Lane 2 : Recombinant Human Ferritin Heavy Chain protein (ab78877) at 0.01 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa


    Exposure time: 30 seconds

References (33)

Publishing research using ab65080? Please let us know so that we can cite the reference in this datasheet.

ab65080 has been referenced in 33 publications.

  • Liu P  et al. Ferrostatin-1 alleviates lipopolysaccharide-induced acute lung injury via inhibiting ferroptosis. Cell Mol Biol Lett 25:10 (2020). PubMed: 32161620
  • Du J  et al. Identification of Frataxin as a regulator of ferroptosis. Redox Biol 32:101483 (2020). PubMed: 32169822
  • Yoshida M  et al. Involvement of cigarette smoke-induced epithelial cell ferroptosis in COPD pathogenesis. Nat Commun 10:3145 (2019). PubMed: 31316058
  • Li Y  et al. Ischemia-induced ACSL4 activation contributes to ferroptosis-mediated tissue injury in intestinal ischemia/reperfusion. Cell Death Differ N/A:N/A (2019). PubMed: 30737476
  • Redinus K  et al. An Hb-mediated circulating macrophage contributing to pulmonary vascular remodeling in sickle cell disease. JCI Insight 4:N/A (2019). PubMed: 31391342
View all Publications for this product

Images

  • Western blot - Anti-Ferritin Heavy Chain antibody (ab65080)
    Western blot - Anti-Ferritin Heavy Chain antibody (ab65080)
    Anti-Ferritin Heavy Chain antibody (ab65080) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa
    Observed band size: 21 kDa
    Additional bands at: 24 kDa (possible post-translational modification)


    Exposure time: 3 minutes


    Ferritin Heavy Chain contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a slightly higher than predicted molecular weight (24 kDa).
  • Immunocytochemistry/ Immunofluorescence - Anti-Ferritin Heavy Chain antibody (ab65080)
    Immunocytochemistry/ Immunofluorescence - Anti-Ferritin Heavy Chain antibody (ab65080)
    ICC/IF image of ab65080 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65080, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody (ab65080)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Heavy Chain antibody (ab65080)
    IHC image of ab65080 staining in Human Normal Liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65080, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Western blot - Anti-Ferritin Heavy Chain antibody (ab65080)
    Western blot - Anti-Ferritin Heavy Chain antibody (ab65080)
    All lanes : Anti-Ferritin Heavy Chain antibody (ab65080) at 1 µg/ml

    Lane 1 : Recombinant Human Ferritin Heavy Chain protein (ab78877) at 0.1 µg
    Lane 2 : Recombinant Human Ferritin Heavy Chain protein (ab78877) at 0.01 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa


    Exposure time: 30 seconds

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