Anti-ROMO1 antibody [OTI2C12] (ab236409)
![Anti-ROMO1 antibody [OTI2C12] (ab236409)](https://www.abcam.com/ps/products/236/ab236409/Images/ab236409-312661-anti-romo1-antibody-oti2c12-western-blot.jpg "Anti-ROMO1 antibody [OTI2C12] (ab236409)")
Key features and details
- Mouse monoclonal [OTI2C12] to ROMO1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Dog, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-ROMO1 antibody [OTI2C12] -
Description
Mouse monoclonal [OTI2C12] to ROMO1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanAfrican green monkeyIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human ROMO1 aa 1 to the C-terminus. (NP_542786) produced in HEK-293T cells.
Database link: P60602 -
Positive control
- WB: HepG2, HeLa, SVT2, A549, COS-7, Jurkat and MDCK whole cell lysates; pCMV6-ENTRY ROMO1 transfected HEK-293T whole cell lysate. IHC-P: Human breast, breast adenocarcinoma, colon adenocarcinoma, ovary adenocarcinoma, endometrial adenocarcinoma, normal pancreas, thyroid carcinoma, normal prostate and normal kidney tissue. ICC/IF: COS-7 and HeLa cells.
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General notes
Clone OTI2C12 (formerly 2C12)
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI2C12 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-ROMO1 antibody [OTI2C12] (ab236409)All lanes : Anti-ROMO1 antibody [OTI2C12] (ab236409) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control, whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY ROMO1, whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 8 kDa
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![Western blot - Anti-ROMO1 antibody [OTI2C12] (ab236409)](https://www.abcam.com/ps/products/236/ab236409/Images/ab236409-312662-anti-romo1-antibody-oti2c12-western-blot.jpg)
Western blot - Anti-ROMO1 antibody [OTI2C12] (ab236409)All lanes : Anti-ROMO1 antibody [OTI2C12] (ab236409) at 1/2000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : SV-T2 (Mouse cell line) whole cell lysate
Lane 4 : A549 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 7 : MDCK (canine kidney cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 8 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)](https://www.abcam.com/ps/products/236/ab236409/Images/ab236409-312663-anti-romo1-antibody-oti2c12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)Paraffin-embedded human breast tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)](https://www.abcam.com/ps/products/236/ab236409/Images/ab236409-312664-anti-romo1-antibody-oti2c12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)Paraffin-embedded human breast adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)](https://www.abcam.com/ps/products/236/ab236409/Images/ab236409-312665-anti-romo1-antibody-oti2c12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)Paraffin-embedded human colon adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)](https://www.abcam.com/ps/products/236/ab236409/Images/ab236409-312666-anti-romo1-antibody-oti2c12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)Paraffin-embedded human kidney tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)](https://www.abcam.com/ps/products/236/ab236409/Images/ab236409-312667-anti-romo1-antibody-oti2c12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)Paraffin-embedded human ovary adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)](https://www.abcam.com/ps/products/236/ab236409/Images/ab236409-312668-anti-romo1-antibody-oti2c12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)Paraffin-embedded human pancreas tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)](https://www.abcam.com/ps/products/236/ab236409/Images/ab236409-312670-anti-romo1-antibody-oti2c12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)Paraffin-embedded human thyroid tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)](https://www.abcam.com/ps/products/236/ab236409/Images/ab236409-312671-anti-romo1-antibody-oti2c12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)Paraffin-embedded human endometrial adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)](https://www.abcam.com/ps/products/236/ab236409/Images/ab236409-312672-anti-romo1-antibody-oti2c12-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)Paraffin-embedded human prostate tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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![Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (ab236409)](https://www.abcam.com/ps/products/236/ab236409/Images/ab236409-312673-anti-romo1-antibody-oti2c12-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (ab236409)COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY ROMO1 stained for ROMO1 (green) using ab236409 at 1/100 dilution in ICC/IF.
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![Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (ab236409)](https://www.abcam.com/ps/products/236/ab236409/Images/ab236409-312674-anti-romo1-antibody-oti2c12-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (ab236409)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ROMO1 (green) using ab236409 at 1/100 dilution in ICC/IF.