Antibodies, Proteins, Kits and Reagents for Life Science

341 740 ₸ Product size

100 µl 341 740 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4476] to EPF
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Human

Product name

Anti-EPF antibody [EPR4476]
See all EPF primary antibodies
Description

Rabbit monoclonal [EPR4476] to EPF
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A431, Neuro-2a, A549, and MCF7 cell lysates IHC-P: Human colon and kidney tissues ICC/IF: HeLa cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4476
Isotype

IgG
Research areas

Western blot - Anti-EPF antibody [EPR4476] (ab108600)

All lanes : Anti-EPF antibody [EPR4476] (ab108600) at 1/10000 dilution

Lane 1 : A431 cell lysate
Lane 2 : Neuro-2a cell lysate
Lane 3 : A549 cell lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 11 kDa
Observed band size: 11 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (ab108600)

Immunohistochemical staining of EPF in paraffin embedded Human colon tissue, using ab108600 at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (ab108600)

Immunohistochemical staining of EPF in paraffin embedded Human kidney tissue, using ab108600 at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-EPF antibody [EPR4476] (ab108600)

Immunofluorescent staining of EPF in HeLa cells, using ab108600 at a dilution of 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (ab108600)

ab108600 showing positive staining in Normal uterus tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (ab108600)

ab108600 showing positive staining in Ovarian carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (ab108600)

ab108600 showing positive staining in Normal breast tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (ab108600)

ab108600 showing positive staining in Colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (ab108600)

ab108600 showing positive staining in Normal stomach tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-EPF antibody [EPR4476] (ab108600)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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