Anti-Hsp70 antibody (ab231128)
Key features and details
- Rabbit polyclonal to Hsp70
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Hsp70 antibody
See all Hsp70 primary antibodies -
Description
Rabbit polyclonal to Hsp70 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human, Pig
Predicted to work with: Rat, Cow, Dog, Orangutan
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Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human Hsp70 aa 250 to the C-terminus. (Expressed in E.coli).
Database link: P0DMV8 -
Positive control
- IHC-P: Human stomach, brain, prostate, kidney breast cancer and prostate cancer tissues. WB: Pig liver and brain lysates; Mouse liver lysate; A549 cell lysate; Recombinant human Hsp70 protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab231128 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody (ab231128)
Paraffin-embedded human stomach tissue stained for Hsp70 using ab231128 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody (ab231128)Paraffin-embedded human brain tissue stained for Hsp70 using ab231128 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody (ab231128)Paraffin-embedded human prostate tissue stained for Hsp70 using ab231128 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody (ab231128)Paraffin-embedded human kidney tissue stained for Hsp70 using ab231128 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody (ab231128)Paraffin-embedded human breast cancer tissue stained for Hsp70 using ab231128 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody (ab231128)Paraffin-embedded human prostate cancer tissue stained for Hsp70 using ab231128 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-Hsp70 antibody (ab231128)Anti-Hsp70 antibody (ab231128) at 2 µg/ml + Recombinant human Hsp70 protein
Predicted band size: 70 kDa
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Western blot - Anti-Hsp70 antibody (ab231128)All lanes : Anti-Hsp70 antibody (ab231128) at 2 µg/ml
Lane 1 : Pig liver lysate
Lane 2 : Pig brain lysate
Lane 3 : Mouse liver lysate
Lane 4 : A549 (human lung carcinoma cell line) cell lysate
Predicted band size: 70 kDa
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Western blot - Anti-Hsp70 antibody (ab231128)All lanes : Anti-Hsp70 antibody (ab231128) at 2 µg/ml
Lane 1 : Wild-type A549 (human lung carcinoma cell line) cell lysate
Lane 2 : Hsp70 knockout A549 (human lung carcinoma cell line) cell lysate
Predicted band size: 70 kDa
