Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hsp70 antibody [EPR16893] - BSA and Azide free (ab238947)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16893] to Hsp70 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Hsp70 antibody [EPR16893] - BSA and Azide free
    See all Hsp70 primary antibodies

  • Description

    Rabbit monoclonal [EPR16893] to Hsp70 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse kidney tissue.

  • General notes

    Ab238947 is the carrier-free version of ab194360. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238947 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-Hsp70 antibody [EPR16893] - BSA and Azide free (ab238947)
    Flow Cytometry - Anti-Hsp70 antibody [EPR16893] - BSA and Azide free (ab238947)

    Flow cytometry analysis of HeLa cells labelling Hsp70 (red) with purified ab194360 at dilution of 1/120. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab194360).

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [EPR16893] - BSA and Azide free (ab238947)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody [EPR16893] - BSA and Azide free (ab238947)

    Immunofluorescent analysis of A431 cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling Hsp70 with ab194360 at 1/150 dilution (11 μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab194360).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [EPR16893] - BSA and Azide free (ab238947)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp70 antibody [EPR16893] - BSA and Azide free (ab238947)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Hsp70 with ab194360 at 1/1000 (1.67μg/mL) dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab194360).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Hsp70 antibody [EPR16893] - BSA and Azide free (ab238947)
    Anti-Hsp70 antibody [EPR16893] - BSA and Azide free (ab238947)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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