Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19275] to DRP1 - BSA and Azide free
  • Suitable for: Flow Cyt, IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DRP1 antibody [EPR19275] - BSA and Azide free
    See all DRP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19275] to DRP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250742 is the carrier-free version of ab184248. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250742 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)
    Western blot - Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)
    All lanes : Anti-DRP1 antibody [EPR19275] (ab184248) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa


    Exposure time: 30 seconds


    This data was developed using ab184248, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)

    This data was developed using ab184248, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling DRP1 with ab184248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human cerebellum [PMID: 9422767]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)

    This data was developed using ab184248, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling DRP1 with ab184248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human glioma [PMID: 25730670]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)
    Western blot - Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)
    All lanes : Anti-DRP1 antibody [EPR19275] (ab184248) at 1/1000 dilution

    Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 5 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
    Lane 6 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 8 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 9 : U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa



    This data was developed using ab184248, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST. Exposure times: Lanes 1-8: 3 minutes; Lane 9: 30 seconds.

  • Western blot - Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)
    Western blot - Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)
    All lanes : Anti-DRP1 antibody [EPR19275] (ab184248) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse spleen tissue lysate
    Lane 4 : Rat brain tissue lysate
    Lane 5 : Rat heart tissue lysate
    Lane 6 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa



    This data was developed using ab184248, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1 and 4: 1 second; Lane 2: 3 seconds; Lane 3: 10 seconds; Lanes 5-6: 5 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)
    This data was developed using ab184248, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling DRP1 with ab184248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse cerebellum [PMID: 9422767]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)
    This data was developed using ab184248, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling DRP1 with ab184248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat cerebellum [PMID: 9422767]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)
    Flow Cytometry - Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)
    This data was developed using ab184248, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling DRP1 with ab184248 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
  • Immunoprecipitation - Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)
    Immunoprecipitation - Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)

    This data was developed using ab184248, the same antibody clone in a different buffer formulation.

    DRP1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab184248 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184248 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

     Lane 1: HeLa whole cell lysate, 10 µg (Input).

     Lane 2: ab184248 IP in HeLa whole cell lysate.

     Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184248 in HeLa whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)
    Anti-DRP1 antibody [EPR19275] - BSA and Azide free (ab250742)

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