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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-NEK7 antibody (ab95873)

Anti-NEK7 antibody (ab95873)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to NEK7
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-NEK7 antibody
    See all NEK7 primary antibodies
  • Description

    Rabbit polyclonal to NEK7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Orangutan
  • Immunogen

    A region between residue 1 and 50 of human NEK7 (NP_598001.1)

  • Positive control

    • HeLa and 293T cell lysates
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab95873 was affinity purified using an epitope specific to NEK7 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Cytokinesis
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies

Images

  • Western blot - Anti-NEK7 antibody (ab95873)
    Western blot - Anti-NEK7 antibody (ab95873)
    All lanes : Anti-NEK7 antibody (ab95873) at 0.4 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Predicted band size: 35 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK7 antibody (ab95873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK7 antibody (ab95873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling NEK7 with ab95873 at 1/1000 (1µg/ml). Detection: DAB.
  • Immunoprecipitation - Anti-NEK7 antibody (ab95873)
    Immunoprecipitation - Anti-NEK7 antibody (ab95873)
    Detection of NEK7 by Western Blot of Immunprecipitate. (1) ab95873 at 0.4µg/ml staining NEK7 which had been immunoprecipitated from HeLa whole cell lysate using ab95873 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane), (2) control IgG. Detection: Chemiluminescence with exposure time of 30 seconds.

    All lanes : Anti-NEK7 antibody (ab95873) at 1 µg/ml

    Lane 1 : IP: ab95873 at 10µg/mg lysate
    Lane 2 : IP: Controle IgG at 10µg/mg lysate

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-NEK7 antibody (ab95873)

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    Anti-NEK7 antibody [EPR4901] (ab109433)

    Applications: Flow Cyt, WB

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    Anti-NEK7 antibody [EPR4900] - BSA and Azide free (ab240065)

    Applications: IP, WB

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    Anti-NEK7 antibody (ab166776)

    Applications: IHC-P, WB

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    Anti-NEK7 antibody [EPR4900] (ab133514)

    Applications: IP, WB

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    Anti-NEK7 antibody (ab68060)

    Applications: WB

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    Anti-NEK7 antibody (ab96538)

    Applications: ICC/IF, WB

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