Antibodies, Proteins, Kits and Reagents for Life Science

355 142 ₸ Product size

100 µl 355 142 ₸ 10 µl 140 716 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4900] to NEK7
Suitable for: WB, IP
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-NEK7 antibody [EPR4900]
See all NEK7 primary antibodies
Description

Rabbit monoclonal [EPR4900] to NEK7
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Mouse

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to residues in Human NEK7 (UniProt ID: Q8TDX7)
Positive control
- Jurkat, A549, C6, RAW264.7, PC12 and NIH/3T3 cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IP	Mouse
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4900
Isotype

IgG
Research areas

Immunoprecipitation - Anti-NEK7 antibody [EPR4900] (ab133514)

ab133514 immunoprecipitating NEK7. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/1000.

Lane 1: H/3T3 (Mouse embryonic fibroblast) whole cell lysate 10ug
Lane 2: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab133514 in NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Western blot - Anti-NEK7 antibody [EPR4900] (ab133514)

All lanes : Anti-NEK7 antibody [EPR4900] (ab133514) at 1/10000 dilution

Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 35 kDa
Western blot - Anti-NEK7 antibody [EPR4900] (ab133514)

Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: NEK7 knockout HAP1 cell lysate (40 µg)
Lane 3: A549 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133514 observed at 35 kDa. Red - loading control, ab18058, observed at 124 kDa.

ab133514 was shown to specifically react with NEK7 when NEK7 knockout samples were used. Wild-type and NEK7 knockout samples were subjected to SDS-PAGE. Ab133514 and ab18058 (loading control to Vinculin) were diluted at 1/10000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-NEK7 antibody [EPR4900] (ab133514)

All lanes : Anti-NEK7 antibody [EPR4900] (ab133514) at 1/10000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : A549 cell lysate
Lane 3 : C6 cell lysate
Lane 4 : RAW264.7 cell lysate
Lane 5 : PC12 cell lysate
Lane 6 : NIH/3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 35 kDa
Observed band size: 32 kDa
why is the actual band size different from the predicted?
Anti-NEK7 antibody [EPR4900] (ab133514)

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