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Anti-LAMTOR2 antibody [EPR14378] (ab183514)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14378] to LAMTOR2
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-LAMTOR2 antibody [EPR14378]
    See all LAMTOR2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14378] to LAMTOR2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2, Raji and LnCaP cell lysates. IHC-P: Human thyroid tissue and Human gastric carcinoma tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-LAMTOR2 antibody [EPR14378] (ab183514)
    Western blot - Anti-LAMTOR2 antibody [EPR14378] (ab183514)
    All lanes : Anti-LAMTOR2 antibody [EPR14378] (ab183514) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : LAMTOR2 knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : LNCaP cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 14 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab183514 observed at 14 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab183514 Anti-LAMTOR2 antibody [EPR14378] was shown to specifically react with LAMTOR2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265828 (knockout cell lysate ab258020) was used. Wild-type and LAMTOR2 knockout samples were subjected to SDS-PAGE. ab183514 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMTOR2 antibody [EPR14378] (ab183514)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMTOR2 antibody [EPR14378] (ab183514)

    Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma labeling LAMTOR2 using ab183514 at a 1/50 dilution, using a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMTOR2 antibody [EPR14378] (ab183514)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMTOR2 antibody [EPR14378] (ab183514)

    Immunohistochemical analysis of paraffin-embedded Human thyroid tissue labeling LAMTOR2 using ab183514 at a 1/50 dilution, using a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-LAMTOR2 antibody [EPR14378] (ab183514)
    Western blot - Anti-LAMTOR2 antibody [EPR14378] (ab183514)
    All lanes : Anti-LAMTOR2 antibody [EPR14378] (ab183514) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : LnCaP cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 14 kDa

  • Anti-LAMTOR2 antibody [EPR14378] (ab183514)
    Anti-LAMTOR2 antibody [EPR14378] (ab183514)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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