Anti-Glypican 1/ GPC1 antibody (ab73979)
Key features and details
- Rabbit polyclonal to Glypican 1/ GPC1
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glypican 1/ GPC1 antibody
See all Glypican 1/ GPC1 primary antibodies -
Description
Rabbit polyclonal to Glypican 1/ GPC1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide derived from the N-terminal domain of human Glypican/GPC1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody (ab73979)ICC/IF image of ab73979 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73979, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
