Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19285] to Glypican 1/ GPC1
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-Glypican 1/ GPC1 antibody [EPR19285]
See all Glypican 1/ GPC1 primary antibodies
Description

Rabbit monoclonal [EPR19285] to Glypican 1/ GPC1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MDA-MB-231, MOLT-4, HepG2, K562, SH-SY5Y and MCF7 whole cell lysates; Human fetal kidney, colon and fetal spleen lysates. ICC/IF: MCF7 and MDA-MB-231 cells. Flow Cyt: MDA-MB-231 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19285
Isotype

IgG
Research areas

Western blot - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)

All lanes : Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343) at 1/1000 dilution

Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 5 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 61 kDa
Additional bands at: 55 kDa (possible post-translational modification)

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with the literature (PMID: 9802880).
Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing membrane associated and cytoplasmic staining on MDA-MB-231 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on MCF7 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
Flow Cytometry - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)

Flow cytometric analysis of 4% paraformaldehyde-fixed MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/60 dilution (red) compared with a Rabbit IgG,monoclonal[EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti Rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)

All lanes : Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343) at 1/1000 dilution

Lane 1 : Human fetal kidney lysate
Lane 2 : Human colon lysate
Lane 3 : Human fetal spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 61 kDa
Observed band size: 55 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 5 seconds; Lanes 2 and 3: 10 seconds.
Anti-Glypican 1/ GPC1 antibody [EPR19285] (ab199343)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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