Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19285] to Glypican 1/ GPC1 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free
    See all Glypican 1/ GPC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19285] to Glypican 1/ GPC1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MDA-MB-231, MOLT-4, HepG2, K562, SH-SY5Y and MCF7 whole cell lysates; Human fetal kidney, colon and fetal spleen lysates. ICC/IF: MCF7 and MDA-MB-231 cells. Flow Cyt: MDA-MB-231 cells.

  • General notes

    ab271962 is the carrier-free version of ab199343. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)
    Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing membrane associated and cytoplasmic staining on MDA-MB-231 cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199343).
  • Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)
    Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on MCF7 cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199343).
  • Flow Cytometry - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)
    Flow Cytometry - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)

    Flow cytometric analysis of 4% paraformaldehyde-fixed MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/60 dilution (red) compared with a Rabbit IgG,monoclonal[EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199343).
  • Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)
    Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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