Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)
![Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)](https://www.abcam.com/ps/products/271/ab271962/Images/ab271962-2-ab199343266586antiglypican1gpc1antibodyepr19285immunofluorescence.jpg "Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19285] to Glypican 1/ GPC1 - BSA and Azide free
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free
See all Glypican 1/ GPC1 primary antibodies -
Description
Rabbit monoclonal [EPR19285] to Glypican 1/ GPC1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MDA-MB-231, MOLT-4, HepG2, K562, SH-SY5Y and MCF7 whole cell lysates; Human fetal kidney, colon and fetal spleen lysates. ICC/IF: MCF7 and MDA-MB-231 cells. Flow Cyt: MDA-MB-231 cells.
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General notes
ab271962 is the carrier-free version of ab199343. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19285 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing membrane associated and cytoplasmic staining on MDA-MB-231 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199343). -
![Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)](https://www.abcam.com/ps/products/271/ab271962/Images/ab271962-3-ab199343266587antiglypican1gpc1antibodyepr19285immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on MCF7 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199343). -
![Flow Cytometry - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)](https://www.abcam.com/ps/products/271/ab271962/Images/ab271962-1-ab199343266585antiglypican1gpc1antibodyepr19285flowcytometry.jpg)
Flow Cytometry - Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)Flow cytometric analysis of 4% paraformaldehyde-fixed MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Glypican 1/ GPC1 with ab199343 at 1/60 dilution (red) compared with a Rabbit IgG,monoclonal[EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199343). -
![Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)](https://www.abcam.com/ps/products/271/ab271962/Images/ab271962-4-benefits-of-recombinant-antibodies.png)
Anti-Glypican 1/ GPC1 antibody [EPR19285] - BSA and Azide free (ab271962)
