Human CSPG4 ELISA Kit (NG2/MCSP) (ab267807)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 25.5 pg/ml
- Range: 156.25 pg/ml - 10000 pg/ml
- Sample type: Cell culture media, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CSPG4 ELISA Kit (NG2/MCSP)
See all NG2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.5% Inter-assay Sample n Mean SD CV% Serum 3 0.5% -
Sample type
Serum, Cell culture media, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
25.5 pg/ml -
Range
156.25 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 114 113% - 115% Cell culture media 100 100% - 101% Cit plasma 106 103% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CSPG4 ELISA Kit (NG2/MCSP) (ab267807) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CSPG4 (NG2/MCSP) protein in serum, cit plasma, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CSPG4 (NG2/MCSP) with 25.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Chondroitin sulfate proteoglycan 4 (CSPG4), also known as NG2 or MCSP, is a multifunctional cell surface type I transmembrane protein that is covalently modified with chondroitin sulfate glycosaminoglycan. CSPG4 has been observed to promote epidermal stem cell patterned clustering and used as a cell surface marker for melanoma and glial precursor cells. Studies have associate CSPG4 as important role in facilitating the growth and survival of malignant melanoma as well as migration, invasion, metastasis, and chemoresistance.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CSPG4 (NG2/MCSP) Capture Antibody 1 x 600µl 10X Human CSPG4 (NG2/MCSP) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human CSPG4 (NG2/MCSP) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. -
Tissue specificity
Detected only in malignant melanoma cells. -
Sequence similarities
Contains 15 CSPG (NG2) repeats.
Contains 2 laminin G-like domains. -
Post-translational
modificationsO-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
Phosphorylation by PRKCA regulates its subcellular location and function in cell motility. -
Cellular localization
Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes. - Information by UniProt
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Alternative names
- 4732461B14Rik
- AN2
- AN2 proteoglycan
see all -
Database links
- Entrez Gene: 1464 Human
- Omim: 601172 Human
- SwissProt: Q6UVK1 Human
- Unigene: 513044 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CSPG4 standard curve in Sample Diluent NS. -
Interpolated concentrations of native CSPG4 in human serum and plasma. -
Sandwich ELISA - Human CSPG4 ELISA Kit (NG2/MCSP) (ab267807)To learn more about the advantages of recombinant antibodies see here.
