Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKC theta/PRKCQ antibody [EPR1488Y] - BSA and Azide free (ab247318)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1488Y] to PKC theta/PRKCQ - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PKC theta/PRKCQ antibody [EPR1488Y] - BSA and Azide free
    See all PKC theta/PRKCQ primary antibodies

  • Description

    Rabbit monoclonal [EPR1488Y] to PKC theta/PRKCQ - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects both phosphorylated (pT538) and unphosphorylated PKC theta/PRKCQ.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247318 is the carrier-free version of ab52494 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247318 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as PKC theta

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PKC theta/PRKCQ antibody [EPR1488Y] - BSA and Azide free (ab247318)
    Western blot - Anti-PKC theta/PRKCQ antibody [EPR1488Y] - BSA and Azide free (ab247318)
    All lanes : Anti-PKC theta/PRKCQ antibody [EPR1488Y] (ab52494) at 1/1000 dilution (purified)

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat spleen tissue lysate
    Lane 3 : C2C12 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution

    Predicted band size: 85 kDa
    Observed band size: 78 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab52494, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PKC theta/PRKCQ antibody [EPR1488Y] - BSA and Azide free (ab247318)
    Western blot - Anti-PKC theta/PRKCQ antibody [EPR1488Y] - BSA and Azide free (ab247318)
    Anti-PKC theta/PRKCQ antibody [EPR1488Y] (ab52494) at 1/1000 dilution (purified) + Jurkat whole cell lysate at 10 µg

    Secondary
    Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution

    Predicted band size: 85 kDa
    Observed band size: 78 kDa why is the actual band size different from the predicted?



    This data was developed using ab52494, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PKC theta/PRKCQ antibody [EPR1488Y] - BSA and Azide free (ab247318)
    Western blot - Anti-PKC theta/PRKCQ antibody [EPR1488Y] - BSA and Azide free (ab247318)
    Anti-PKC theta/PRKCQ antibody [EPR1488Y] (ab52494) at 1/500 dilution (unpurified) + Jurkat cell lysate at 10 µg

    Secondary
    HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 85 kDa
    Observed band size: 85 kDa



    This data was developed using ab52494, the same antibody clone in a different buffer formulation.

     

  • Anti-PKC theta/PRKCQ antibody [EPR1488Y] - BSA and Azide free (ab247318)
    Anti-PKC theta/PRKCQ antibody [EPR1488Y] - BSA and Azide free (ab247318)

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