Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22277] to PKC theta/PRKCQ
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-PKC theta/PRKCQ antibody [EPR22277]
    See all PKC theta/PRKCQ primary antibodies

  • Description

    Rabbit monoclonal [EPR22277] to PKC theta/PRKCQ

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, MOLT-4 and K562 whole cell lysate. IHC-P: Human tonsil tissue. Human gastrointestinal stromal tumor (GIST). IP: PKC theta/PRKCQ IP in K562 whole cell lysate.

  • General notes

     This product was previously labelled as PKC theta

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)

    Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor (GIST) tissue labeling PKC theta/PRKCQ with ab230971 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human gastrointestinal stromal tumor (PMID: 15289315) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PKC theta/PRKCQ with ab230971 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in T lymphocytes in tonsil (PMID: 27149922) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)

    Immunohistochemical analysis of paraffin-embedded human leiomyosarcoma tissue labeling PKC theta/PRKCQ with ab230971 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Negative control: No staining in human leiomyosarcoma (PMID: 15289315).

    Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)
    Western blot - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)
    All lanes : Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 2 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate
    Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 82 kDa
    Observed band size: 75, 40 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in the literature. The 40kDa band is a cleaved form (PMID: 9252332, PMID: 24924627).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 15 seconds; Lanes 2-3: 3 minutes.

  • Immunoprecipitation - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)
    Immunoprecipitation - Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)

    PKC theta/PRKCQ was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab230971 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230971 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: K562 whole cell lysate 10 µg (Input).
    Lane 2: ab230971 IP in K562 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230971 in K562 whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 seconds.

  • Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)
    Anti-PKC theta/PRKCQ antibody [EPR22277] (ab230971)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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