Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] - BSA and Azide free (ab272401)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23152-241] to IL36 alpha/IL-1F6 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] - BSA and Azide free
    See all IL36 alpha/IL-1F6 primary antibodies

  • Description

    Rabbit monoclonal [EPR23152-241] to IL36 alpha/IL-1F6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-Fr or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW 264.7 treated with 1 /ml LPS for 24 hours whole lysate. IHC-P: Mouse esophagus, Human skin and Human esophagus, Rat esophagus tissues. ICC/IF: RAW264.7+LPS cells. Flow Cyt: RAW 264.7 cells.

  • General notes

    ab272401 is the carrier-free version of ab269274. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23152-241

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] - BSA and Azide free (ab272401)
    Flow Cytometry - Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] - BSA and Azide free (ab272401)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 1ug/ml lipopolysaccharide (LPS) for 24h (Red)/ Untreated control (Green) cells labelling IL36 alpha/IL-1F6 with ab269274 at 1/600 dilution (0.1ug) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269274).

  • Immunocytochemistry/ Immunofluorescence - Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] - BSA and Azide free (ab272401)
    Immunocytochemistry/ Immunofluorescence - Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] - BSA and Azide free (ab272401)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW264.7+LPS(1ug/ml, 24h)(-/+) cells labelling IL36 alpha/IL-1F6 with ab269274 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (Green). Confocal image showing weak cytoplasmic and strong nuclear staining in RAW 264.7 cells treated with lipopolysaccharide (LPS, 1 ug/ml) for 24 hours. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269274).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] - BSA and Azide free (ab272401)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] - BSA and Azide free (ab272401)

    Immunohistochemical analysis of paraffin-embedded Rat esophagus tissue labeling IL36 alpha/IL-1F6 with ab269274 at 500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat esophagus. The section was incubated with ab269274 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269274).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] - BSA and Azide free (ab272401)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] - BSA and Azide free (ab272401)

    Immunohistochemical analysis of paraffin-embedded Human esophagus tissue labeling IL36 alpha/IL-1F6 with ab269274 at 500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human esophagus. The section was incubated with ab269274 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269274).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] - BSA and Azide free (ab272401)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] - BSA and Azide free (ab272401)

    Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling IL36 alpha/IL-1F6 with ab269274 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human skin. The section was incubated with ab269274 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269274).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] - BSA and Azide free (ab272401)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] - BSA and Azide free (ab272401)

    Immunohistochemical analysis of paraffin-embedded Mouse esophagus tissue labeling IL36 alpha/IL-1F6 with ab269274 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse esophagus (PMID: 20101239). The section was incubated with ab269274 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269274).

  • Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] - BSA and Azide free (ab272401)
    Anti-IL36 alpha/IL-1F6 antibody [EPR23152-241] - BSA and Azide free (ab272401)

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