Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FRA1 antibody [EPR23767-177] - BSA and Azide free (ab278103)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23767-177] to FRA1 - BSA and Azide free
  • Suitable for: IP, WB, ICC, Flow Cyt (Intra)
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-FRA1 antibody [EPR23767-177] - BSA and Azide free
    See all FRA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23767-177] to FRA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, ICC, Flow Cyt (Intra)more details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa starved for 24 hours then treated with 100 ng/mL TPA whole cell lysate; NIH/3T3 starved for 2 hours then treated with 100 ng/mL TPA for 4 hours whole cell lysate; MDA-MB-231 whole cell lysate. ICC: HeLa cells. Flow cyt: HeLa cells starved for 24 hours then treated with 100 ng/ml TPA for 4 hours. IP: HeLa staved for 24 hours then treated with 100 ng/mL TPA for 4 hours.

  • General notes

    ab278103 is the carrier-free version of ab252421. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278103 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-FRA1 antibody [EPR23767-177] - BSA and Azide free (ab278103)
    Western blot - Anti-FRA1 antibody [EPR23767-177] - BSA and Azide free (ab278103)
    All lanes : Anti-FRA1 antibody [EPR23767-177] (ab252421) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) staved for 24 hours, whole cell lysate
    Lane 2 : HeLa staved for 24 hours then treated with 100 ng/mL TPA (ab120297) for 4 hours, whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) staved for 2 hours, whole cell lysate
    Lane 4 : NIH/3T3 staved for 2 hours then treated with 100 ng/mL TPA (ab120297) for 4 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 29 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab252421, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID:13679379).

    The expression of FRA1 proteins is induced by TPA treatment (PMID: 13679379).

    Exposure time: 3 minutes.

  • Western blot - Anti-FRA1 antibody [EPR23767-177] - BSA and Azide free (ab278103)
    Western blot - Anti-FRA1 antibody [EPR23767-177] - BSA and Azide free (ab278103)
    Anti-FRA1 antibody [EPR23767-177] (ab252421) at 1/1000 dilution + MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate at 40 µg

    Secondary
    VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?



    This data was developed using ab252421, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunocytochemistry - Anti-FRA1 antibody [EPR23767-177] - BSA and Azide free (ab278103)
    Immunocytochemistry - Anti-FRA1 antibody [EPR23767-177] - BSA and Azide free (ab278103)

    This data was developed using ab252421, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labelling FRA1 with ab252421 at 1/500 (0.918 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). The expression of FRA1 proteins is induced by TPA treatment (PMID: 13679379). Confocal image showing nuclear staining in HeLa cell line after starvation 24 hours, then treatment with Phorbol-12-myristate-13-acetate for 4h is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000  dilution.

  • Flow Cytometry (Intracellular) - Anti-FRA1 antibody [EPR23767-177] - BSA and Azide free (ab278103)
    Flow Cytometry (Intracellular) - Anti-FRA1 antibody [EPR23767-177] - BSA and Azide free (ab278103)

    This data was developed using ab252421, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human cervix adenocarcinoma epithelial cell) starved for 24 hours then treated with 100 ng/ml TPA for 4 hours (Red) /Untreated control (Green) cells labelling FRA1 with ab252421 at 1/500 dilution (0.1ug) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-FRA1 antibody [EPR23767-177] - BSA and Azide free (ab278103)
    Immunoprecipitation - Anti-FRA1 antibody [EPR23767-177] - BSA and Azide free (ab278103)

    This data was developed using ab252421, the same antibody clone in a different buffer formulation.

    FRA1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) staved for 24 hours then treated with 100 ng/mL TPA for 4 hours, whole cell lysate with ab252421 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252421 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) staved for 24 hours then treated with 100 ng/mL TPA for 4 hours, whole cell lysate 10 ug

    Lane 2: ab252421 IP in HeLa staved for 24 hours then treated with 100 ng/mL TPA for 4 hours whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252421 in HeLa staved for 24 hours then treated with 100 ng/mL TPA for 4 hours whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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