Alexa Fluor® 488 Anti-c-Jun antibody [EP693Y] (ab210013)
![Alexa Fluor® 488 Anti-c-Jun antibody [EP693Y] (ab210013)](https://www.abcam.com/ps/products/210/ab210013/Images/ab210013-390545-anticjunantibodyimmunocytochemistryimmunofluorescencehek293human.jpg "Alexa Fluor® 488 Anti-c-Jun antibody [EP693Y] (ab210013)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EP693Y] to c-Jun
- Suitable for: ICC/IF
- Knockout validated
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Overview
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Product name
Alexa Fluor® 488 Anti-c-Jun antibody [EP693Y]
See all c-Jun primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EP693Y] to c-Jun -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: SW480 cells. ICC/IF KO: HEK293 cells (HEK293-JUN KO used as a negative cell line)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EP693Y -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-c-Jun antibody [EP693Y] (ab210013)
ab210013 staining c-Jun in wild-type HEK293 cells (top panel) and c-Jun knockout HEK293 cells (bottom panel). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab210013 at 1/100 dilution (shown in green) and ab195884 (Rat monoclonal to Tubulin - Alexa Fluor® 647) at 1/100 dilution (shown in red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a high-content analysis system (Perkin Elmer, Operetta CLS™).
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-c-Jun antibody [EP693Y] (ab210013)](https://www.abcam.com/ps/products/210/ab210013/Images/ab210013-263774-anti-c-jun-antibody-ep693y-alexa-fluor-488-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-c-Jun antibody [EP693Y] (ab210013)ab210013 staining c-Jun in SW480 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210013 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in SW480 cells fixed with 100% methanol (5 min).
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![Alexa Fluor® 488 Anti-c-Jun antibody [EP693Y] (ab210013)](https://www.abcam.com/ps/products/210/ab210013/Images/ab210013-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 488 Anti-c-Jun antibody [EP693Y] (ab210013)
