Anti-Histone H4 (mono methyl R3) antibody [RM195] (ab214657)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM195] to Histone H4 (mono methyl R3)
- Suitable for: WB, ICC/IF, ELISA
- Reacts with: Human, Recombinant fragment
Overview
-
Product name
Anti-Histone H4 (mono methyl R3) antibody [RM195]
See all Histone H4 primary antibodies -
Description
Rabbit monoclonal [RM195] to Histone H4 (mono methyl R3) -
Host species
Rabbit -
Specificity
ab214657 reacts to Histone H4 monomethylated at Arginine 3. No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human -
Immunogen
Synthetic peptide corresponding to Human Histone H4 (mono methyl R3).
-
Positive control
- Acid extracts of HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 48% PBS, 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
Purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM195 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-Histone H4 (mono methyl R3) antibody [RM195] (ab214657) at 0.5 µg/ml
Lane 1 : acid extracts of HeLa cells
Lane 2 : recombinant Histone H4
-
Immunocytochemistry of sodium butyrate treated HeLA cells useing ab214657. Actin filaments have been labeled with fluorescein
phalloidin (green). -
ab214657 specifically reacts to Histone H4 mono-methylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arg 3 (R3 ctrl), asymmetric dimethylated Arg 3 (R3me2a) or symmetric dimethylated Arg 3 (R3me2s), and citrullinated Arg 3 (R3Cit) in Histone H4.
-