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Epigenetics and Nuclear Signaling Histones H3 Methylated

Anti-Histone H4 (mono methyl R3) antibody [RM195] (ab214657)

Anti-Histone H4 (mono methyl R3) antibody [RM195] (ab214657)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM195] to Histone H4 (mono methyl R3)
  • Suitable for: WB, ICC/IF, ELISA
  • Reacts with: Human, Recombinant fragment

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Overview

  • Product name

    Anti-Histone H4 (mono methyl R3) antibody [RM195]
    See all Histone H4 primary antibodies
  • Description

    Rabbit monoclonal [RM195] to Histone H4 (mono methyl R3)
  • Host species

    Rabbit
  • Specificity

    ab214657 reacts to Histone H4 monomethylated at Arginine 3. No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Histone H4 (mono methyl R3).

  • Positive control

    • Acid extracts of HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 48% PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM195
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H4
    • Methylated

Images

  • Western blot - Anti-Histone H4 (mono methyl R3) antibody [RM195] (ab214657)
    Western blot - Anti-Histone H4 (mono methyl R3) antibody [RM195] (ab214657)
    All lanes : Anti-Histone H4 (mono methyl R3) antibody [RM195] (ab214657) at 0.5 µg/ml

    Lane 1 : acid extracts of HeLa cells
    Lane 2 : recombinant Histone H4
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (mono methyl R3) antibody [RM195] (ab214657)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (mono methyl R3) antibody [RM195] (ab214657)

    Immunocytochemistry of sodium butyrate treated HeLA cells useing ab214657. Actin filaments have been labeled with fluorescein
    phalloidin (green).

  • ELISA - Anti-Histone H4 (mono methyl R3) antibody [RM195] (ab214657)
    ELISA - Anti-Histone H4 (mono methyl R3) antibody [RM195] (ab214657)

    ab214657 specifically reacts to Histone H4 mono-methylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arg 3 (R3 ctrl), asymmetric dimethylated Arg 3 (R3me2a) or symmetric dimethylated Arg 3 (R3me2s), and citrullinated Arg 3 (R3Cit) in Histone H4.

  • Anti-Histone H4 (mono methyl R3) antibody [RM195] (ab214657)
    Anti-Histone H4 (mono methyl R3) antibody [RM195] (ab214657)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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