Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17906] to Histone H4 (acetyl K12)
  • Suitable for: Flow Cyt, PepArr, WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Histone H4 (acetyl K12) antibody [EPR17906]
    See all Histone H4 primary antibodies

  • Description

    Rabbit monoclonal [EPR17906] to Histone H4 (acetyl K12)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    PepArr
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and NIH/3T3 whole cell lysates treated with 500 ng/ml Trichostatin A for 4 hours. IHC: Human and rat colon ICC/IF: HeLa cells treated with 500 ng/ml Trichostatin A for 4 hours.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17906

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    Western blot - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    All lanes : Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793) at 1/10000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) untreated whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells, treated with Trichostatin A (500 ng/ml) for 4 hours or untreated, labeling Histone H4 (acetyl K12) with ab177793 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line is observed. Acetylation level increased after treatment with Trichostatin A (500 ng/ml) for 4 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    1. ab177793 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Flow Cytometry - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    Flow Cytometry - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) treated (Red)/untreated (Green) with 500ng/ml Trichostatin A for 4 hours with purified ab177793 at 1/80 dilution. The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

  • Peptide Array - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    Peptide Array - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)

    ab177793 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
     
    Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
     
    The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
     

  • Western blot - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    Western blot - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    All lanes : Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793) at 1/10000 dilution

    Lane 1 : NIH/3T3 (Mouse embyro fibroblast cells) treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates
    Lane 2 : NIH/3T3 (Mouse embyro fibroblast cells) untreated whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 11 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H4 (acetyl K12) with ab177793 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Nucleus staining on Human colon tissue is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Histone H4 (acetyl K12) with ab177793 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Nucleus staining on Rat colon tissue is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)
    Anti-Histone H4 (acetyl K12) antibody [EPR17906] (ab177793)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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