Anti-Histone H4 (acetyl K20) antibody [RM205] (ab214655)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM205] to Histone H4 (acetyl K20)
- Suitable for: WB, ICC/IF, ELISA
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-Histone H4 (acetyl K20) antibody [RM205]
See all Histone H4 primary antibodies -
Description
Rabbit monoclonal [RM205] to Histone H4 (acetyl K20) -
Host species
Rabbit -
Specificity
ab214655 reacts to Histone H4 acetylated at Lysine 20. No cross reactivity with other acetylated Lysines in Histone H4. -
Tested Applications & Species
See all applications and species dataApplication Species ELISA HumanICC/IF HumanWB Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).
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Positive control
- Acid extracts of HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 48% PBS, 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
RM205 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Histone H4 (acetyl K20) antibody [RM205] (ab214655) at 1 µg/ml
Lane 1 : acid extracts of HeLa cells
Lane 2 : recombinant Histone H4
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Immunocytochemical analysis of Hela (Human epithelial cell line from cervix adenocarcinoma) labeling Histone H4 (acyl K20) with ab214655 (red).
Counterstained actin with fluorescein phalloidin (green).
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ab214655 specifically reacts to Histone H4 acetylated at Lysine 20 (K20ac). No cross reactivity with unmodified Lysine 20 (K20 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.
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