Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16606] to Histone H4 (acetyl K5 + K8 + K12 + K16)
Suitable for: PepArr, WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606]
See all Histone H4 primary antibodies
Description

Rabbit monoclonal [EPR16606] to Histone H4 (acetyl K5 + K8 + K12 + K16)
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Rat Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa treated with Sodium butyrate extract lysates; NIH/3T3 treated with Trichostatin A whole cell lysate. IHC-P: Human, Mouse and Rat colon tissues. ICC/IF: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16606
Isotype

IgG
Research areas

Western blot - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)

All lanes : Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790) at 1/20000 dilution

Lane 1 : HeLa treated with Sodium butyrate extract lysates
Lane 2 : Untreated HeLa extract lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 11 kDa
Observed band size: 11 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling Histone H4 (acetyl K5 + K8 + K12 + K16) with ab177790 at 1/5000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Nuclear staining on HeLa cell line is observed. The expression increased after treatment with TSA (50 μg/ml) for 4 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
1. ab177790 at 1/15000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H4 (acetyl K5 + K8 + K12 + K16) with ab177790 at 1/1000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of Human colon tissue is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary ab, secondary ab is prediluted Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)

All lanes : Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790) at 1/100000 dilution

Lane 1 : NIH/3T3 treated with Trichostatin A whole cell lysates
Lane 2 : Untreated NIH/3T3 whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 11 kDa
Observed band size: 11 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Peptide Array - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)

ab177790 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Histone H4 (acetyl K5 + K8 + K12 + K16) with ab177790 at 1/1000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of mouse colon tissue is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary ab, secondary ab is prediluted Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Histone H4 (acetyl K5 + K8 + K12 + K16) with ab177790 at 1/1000 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nucleus staining on glandular epithelium of rat colon tissue is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary ab, secondary ab is prediluted Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Histone H4 (acetyl K5 + K8 + K12 + K16) antibody [EPR16606] (ab177790)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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