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Epigenetics and Nuclear Signaling Histones H3 Acetylated

Anti-Histone H3 (acetyl K9, phospho S10) antibody (ab272166)

Anti-Histone H3 (acetyl K9, phospho S10) antibody (ab272166)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Histone H3 (acetyl K9, phospho S10)
  • Suitable for: ICC/IF, WB, Dot blot
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG

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Overview

  • Product name

    Anti-Histone H3 (acetyl K9, phospho S10) antibody
    See all Histone H3 primary antibodies
  • Description

    Rabbit polyclonal to Histone H3 (acetyl K9, phospho S10)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Histone H3 (acetyl K9, phospho S10).
    Database link: Q71DI3

  • Positive control

    • WB: NIH/3T3 cell lysate. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Acetylated

Images

  • Western blot - Anti-Histone H3 (acetyl K9, phospho S10) antibody (ab272166)
    Western blot - Anti-Histone H3 (acetyl K9, phospho S10) antibody (ab272166)
    Anti-Histone H3 (acetyl K9, phospho S10) antibody (ab272166) at 1/400 dilution + NIH/3T3 cell lysate at 35 µg

    Predicted band size: 15 kDa



    Block: 5% BLOTTO overnight at 4°C.

  • Dot Blot - Anti-Histone H3 (acetyl K9, phospho S10) antibody (ab272166)
    Dot Blot - Anti-Histone H3 (acetyl K9, phospho S10) antibody (ab272166)

    Dot Blot analysis of Histone H3 (acetyl K9, phospho S10) using ab272166 at 1/1000 dilution.

    Lane 1: K9-Ac/pS10 unmodified.

    Lane 2: pS10.

    Lane 3: K9-Me1.

    Lane 4: K9-Me2.

    Lane 5: K9-Me3.

    Lane 6: K9-Ac peptides.

    Load: 1, 10, and 100 picomoles of peptide.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9, phospho S10) antibody (ab272166)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9, phospho S10) antibody (ab272166)

    Immunofluorescent analysis of HeLa cells labeling Histone H3 (acetyl K9, phospho S10) using ab272166 at 1/10 dilution (Green). The nuclear counterstain is DAPI (Blue). Alpha tubulin is shown in red.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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