Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Histone H3 (acetyl K27) antibody [EPR21218] (ab213279)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21218] to Histone H3 (acetyl K27)
Suitable for: WB
Reacts with: Mouse, Human

Product name

Anti-Histone H3 (acetyl K27) antibody [EPR21218]
See all Histone H3 primary antibodies
Description

Rabbit monoclonal [EPR21218] to Histone H3 (acetyl K27)
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human Histone H3.
General notes

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Monoclonal
Clone number

EPR21218
Isotype

IgG1
Research areas

Western blot - Anti-Histone H3 (acetyl K27) antibody [EPR21218] (ab213279)

All lanes : Anti-Histone H3 (acetyl K27) antibody [EPR21218] (ab213279)

Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa Triton-nuclear lysate
Lane 3 : HeLa Triton-nuclear Butyrate (-) lysate
Lane 4 : HeLa Triton-nuclear Butyrate (+) lysate
Lane 5 : HeLa Triton-nuclear TSA (-) lysate
Lane 6 : HeLa Triton-nuclear TSA (+) lysate
Lane 7 : NIH3T3 whole cell lysate
Lane 8 : NIH3T3 Triton-nuclear lysate
Lane 9 : NIH3T3 Triton-nuclear TSA (-) lysate
Lane 10 : NIH3T3 Triton-nuclear TSA (+) lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : anti-rabbit antibody conjugated to HRP at 1/50000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 15 kDa

Exposure time: 20 minutes

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab213279 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
Anti-Histone H3 (acetyl K27) antibody [EPR21218] (ab213279)

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