Anti-Histone H3 (acetyl K9 + K14 + K18 + K23 + K27) antibody (ab47915)
Key features and details
- Rabbit polyclonal to Histone H3 (acetyl K9 + K14 + K18 + K23 + K27)
- Suitable for: ChIP, Dot blot, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (acetyl K9 + K14 + K18 + K23 + K27) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (acetyl K9 + K14 + K18 + K23 + K27) -
Host species
Rabbit -
Specificity
This antibody recognizes histone H3 acetylated at lysines 9, 14, 18, 23 or 27 as confirmed by dot blot with non-modified histone H3 peptide or peptides No reaction with non-modified histone H3 peptide as tested by dot blot.
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Tested applications
Suitable for: ChIP, Dot blot, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Human Histone H3 (N terminal) (acetyl K9 + K14 + K18 + K23 + K27).
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General notes
Learn about ChIP assay kits, other ChIP antibodies, protocols and more in the ChIP assay guide.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.035% Sodium azide
Constituents: Whole serum, 30% Glycerol -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-Histone H3 (acetyl K9 + K14 + K18 + K23 + K27) antibody (ab47915) Natisvili et al PLoS One. 2016 Nov 2;11(11):e0165873. doi: 10.1371/journal.pone.0165873. eCollection 2016. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
HP1α delocalises from chromocentres upon proteasome inhibition whereas the histone modifications remain unaffected.
(A) Proteasome inhibition does not affect canonical histone modifications on major and minor satellite repeats. NIH/3T3 (Mouse embryo fibroblast cell line) cells were treated with 20μM MG132 for 4h followed by ChIP–qPCR analysis using antibodies against repressive mark H3K9me3 and activating marks H3K4me3, H3K36me3, and H3ac (ab47915). Data is shown as relative enrichment to H3 with background subtraction. The y-axis scale was adjusted depending on the signal obtained with different antibodies. Error bars = SEM of 3 biological replicates.
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ChIP - Anti-Histone H3 (acetyl K9 + K14 + K18 + K23 + K27) antibody (ab47915)ChIP was performed using a high sensitivity kit with 5 µg of chromatin from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells and 10 µL of Anti-Histone H3 (acetyl K9 + K14 + K18 + K23 + K27) antibody ab47915.
ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as binding events detected per 1000 cells using a normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.
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Western blot - Anti-Histone H3 (acetyl K9 + K14 + K18 + K23 + K27) antibody (ab47915)All lanes : Anti-Histone H3 (acetyl K9 + K14 + K18 + K23 + K27) antibody (ab47915) at 1/2000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, no treatment
Lane 2 : HeLa cells, treatment with sodium butyrate
Lysates/proteins at 10 µg per lane.
Predicted band size: 15 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
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Dot Blot - Anti-Histone H3 (acetyl K9 + K14 + K18 + K23 + K27) antibody (ab47915)ab47915 tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of ab47915. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:1,000. The amount of peptide (picomoles) spotted is indicated next to the row.
Lane 1: acetyl-Lys9 peptide. Lane 2: unmodified Lys9 peptide. Lane 3: acetyl-Lys14 peptide. Lane 4: unmodified Lys14 peptide. Lane 5: acetyl-Lys18 peptide. Lane 6: unmodified Lys18 peptide. Lane 7: acetyl-Lys23 peptide. Lane 8: unmodified Lys23 peptide. Lane 9: acetyl-Lys27 peptide. Lane 10: unmodified Lys27 peptide.
