Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Histone H3 (acetyl K9) antibody [EPR16988] - BSA and Azide free (ab239404)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16988] to Histone H3 (acetyl K9) - BSA and Azide free
Suitable for: ChIP-sequencing, WB, IHC-P, ICC/IF, ChIP, PepArr
Reacts with: Mouse, Rat, Human

Product name

Anti-Histone H3 (acetyl K9) antibody [EPR16988] - BSA and Azide free
See all Histone H3 primary antibodies
Description

Rabbit monoclonal [EPR16988] to Histone H3 (acetyl K9) - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
ChIP	Human
ChIP-seq	Human
ICC/IF	Human
IHC-P	Human
PepArr	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human colon, mouse colon and rat spleen tissues. ICC/IF: HeLa cells. ChIP-seq: Chromatin from HeLa cells.
General notes
Ab239404 is the carrier-free version of ab177177. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab239404 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16988
Isotype

IgG
Research areas

ChIP-sequencing - Anti-Histone H3 (acetyl K9) antibody [EPR16988] - BSA and Azide free (ab239404)

This data was developed using ab177177, the same antibody clone in a different buffer formulation.

Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ HeLa cells and 4 µg of ab177177 [EPR16988]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.

Additional screenshots of mapped reads can be downloaded here.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K9) antibody [EPR16988] - BSA and Azide free (ab239404)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Histone H3 (acetyl K9) with ab177177 at 1/500 dilution, followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) at 1/500 dilution. Nuclear staining on glandular epithelium of Human colon tissue is observed. Counter stained with hematoxylin.

Negative control: PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177177).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Peptide Array - Anti-Histone H3 (acetyl K9) antibody [EPR16988] - BSA and Azide free (ab239404)

ab177177 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177177).
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody [EPR16988] - BSA and Azide free (ab239404)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (acetyl K9) with ab177177 at 1/3000 dilution, followed by Goat anti-rabbit Alexa Fluor^® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image shows nuclear staining on HeLa cell line. The expression increased after treatment with Trichostatin A (500 ng/ml) for 4 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor^®594 secondary antibody) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab177177 at 1/3000 dilution followed by ab150120 (AlexaFluor^®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor^®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177177).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K9) antibody [EPR16988] - BSA and Azide free (ab239404)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Histone H3 (acetyl K9) with ab177177 at 1/500 dilution, followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) at 1/500 dilution. Nuclear staining on rat spleen tissue is observed. Counter stained with hematoxylin.

Negative control: PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177177).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K9) antibody [EPR16988] - BSA and Azide free (ab239404)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Histone H3 (acetyl K9) with ab177177 at 1/500 dilution, followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) at 1/500 dilution. Nuclear staining on glandular epithelium of mouse colon tissue is observed. Counter stained with hematoxylin.

Negative control: PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177177).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ChIP - Anti-Histone H3 (acetyl K9) antibody [EPR16988] - BSA and Azide free (ab239404)

Chromatin was prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab177177 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177177).
Anti-Histone H3 (acetyl K9) antibody [EPR16988] - BSA and Azide free (ab239404)

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