Antibodies, Proteins, Kits and Reagents for Life Science

338 390 ₸ Product size

100 µl 338 390 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17703] to Histone H3 (citrulline R2)
Suitable for: PepArr, WB
Reacts with: Mouse, Human

Product name

Anti-Histone H3 (citrulline R2) antibody [EPR17703]
See all Histone H3 primary antibodies
Description

Rabbit monoclonal [EPR17703] to Histone H3 (citrulline R2)
Host species

Rabbit
Tested Applications & Species

Application Species
PepArr
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MCF7 cells transfected with PADI4; NIH/3T3 cells transfected with PADI4.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
PepArr	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17703
Isotype

IgG
Research areas

Western blot - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843)

All lanes : Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843) at 1/500 dilution

Lane 1 : MCF7 (Human breast adenocarcinoma cell line) cells transfected with vector control
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) cells transfected with PADI4

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

PADI4 catalyzes the citrullination of arginine residues of histone proteins.
Peptide Array - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843)

ab176843 was tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
Western blot - Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843)

All lanes : Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843) at 1/500 dilution

Lane 1 : NIH/3T3 (Mouse embyro fibroblast cells) cells transfected with vector control
Lane 2 : NIH/3T3 (Mouse embyro fibroblast cells) cells transfected with PADI4

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

PADI4 catalyzes the citrullination of arginine residues of histone proteins.
Anti-Histone H3 (citrulline R2) antibody [EPR17703] (ab176843)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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