Antibodies, Proteins, Kits and Reagents for Life Science

300 865 ₸ Product size

100 µl 300 865 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Histone H3 (citrulline R26) antibody [EPR20606] (ab212082)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20606] to Histone H3 (citrulline R26)
Suitable for: WB, Dot blot, ELISA
Reacts with: Mouse, Human

Product name

Anti-Histone H3 (citrulline R26) antibody [EPR20606]
See all Histone H3 primary antibodies
Description

Rabbit monoclonal [EPR20606] to Histone H3 (citrulline R26)
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Human

See all applications and species data
Immunogen

This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293T transfected with GFP-tagged PADI4 (WT) whole cell lysate treated with CaCl2 and Ionomycin; NIH/3T3 transfected with GFP-tagged PADI4 (WT) whole cell lysate treated with CaCl2.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20606
Isotype

IgG
Research areas

Western blot - Anti-Histone H3 (citrulline R26) antibody [EPR20606] (ab212082)

All lanes : Anti-Histone H3 (citrulline R26) antibody [EPR20606] (ab212082) at 1/1000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (control) then treated with 10 mM CaCl2 and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 2 : HEK-293T transfected with a GFP-tagged PADI4 expression vector then treated with 10 mM CaCl2 and 10 µM Ionomycin for 2 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 5 seconds

Histone H3R26 is citrullinated by PADI4, CaCl₂ is used as a co-factor according to the literature (PMID: 16567635). Ionomycin is used to improve the modification by PADI4 according to the literature (PMID: 26360112).

Blocking/Dilution buffer: 5% BSA/TBST.
Dot Blot - Anti-Histone H3 (citrulline R26) antibody [EPR20606] (ab212082)

Dot blot analysis of Histone H3 (citrulline R26) labeled with ab212082 at 1/1000 dilution.

Lane 1: Histone H3 (citrulline R26) peptide (aa25-36)

Lane 2: Histone H3 (Citrulline R17) peptide (aa1-21)

Lane 3: Histone H3 (Citrulline R8) peptide (aa1-21)

Lane 4: Histone H3 (Citrulline R2) peptide (aa1-21)

Lane 5: Histone H3 peptide (aa1-21)

Lane 6: Histone H3 peptide (aa19-36)

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 30 seconds.
Western blot - Anti-Histone H3 (citrulline R26) antibody [EPR20606] (ab212082)

All lanes : Anti-Histone H3 (citrulline R26) antibody [EPR20606] (ab212082) at 1/1000 dilution

Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) transfected with a GFP-tagged empty vector (control) then treated with 10 mM CaCl2 for 2 hours, whole cell lysate
Lane 2 : NIH/3T3 transfected with a GFP-tagged PADI4 expression vector treated with 10 mM CaCl2 for 2 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 3 minutes

Histone H3R26 is citrullinated by PADI4, CaCl₂ is used as a co-factor according to the literature (PMID: 16567635). Ionomycin is used to improve the modification by PADI4 according to the literature (PMID: 26360112).

Blocking/Dilution buffer: 5% BSA/TBST.
ELISA - Anti-Histone H3 (citrulline R26) antibody [EPR20606] (ab212082)

ELISA using ab212082 labeling Histone H3 (Citrulline R26), Histone H3R26 unmodified, Histone H3 (Citrulline R17), Histone H3 (Citrulline R8), Histone H3 (Citrulline R2) and Histone H3R2/8/17 unmodified at 1 µg/ml. ab212082 was used at a range between 0 -1 µg/ml.

Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) was used as secondary antibody at 1/2500 dilution.

ab212082 cross binds H3R8Cit at 14%.
Anti-Histone H3 (citrulline R26) antibody [EPR20606] (ab212082)

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