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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Cardiovascular Atherosclerosis Thrombosis Platelets

Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] - BSA and Azide free (ab221924)

Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] - BSA and Azide free (ab221924)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1978Y] to COX2 / Cyclooxygenase 2 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] - BSA and Azide free
    See all COX2 / Cyclooxygenase 2 primary antibodies
  • Description

    Rabbit monoclonal [EP1978Y] to COX2 / Cyclooxygenase 2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab213704)

  • Positive control

    • Raw264.7 cell lysate + LPS. Flow Cyt: HeLa cells
  • General notes

    ab221924 is the carrier-free version of ab62331 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab221924 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 1.01 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1978Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Prostaglandins
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Heart disease

Images

  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] - BSA and Azide free (ab221924)
    Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] - BSA and Azide free (ab221924)
    All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/1000 dilution (purified)

    Lane 1 : Untreated Raw 264.7 cell lysate
    Lane 2 : Raw 264.7 treated with LPS

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 69 kDa
    Observed band size: 72 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab62331, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] - BSA and Azide free (ab221924)
    Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] - BSA and Azide free (ab221924)
    All lanes : Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/1000 dilution (Purified)

    Lane 1 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution

    Predicted band size: 69 kDa
    Observed band size: 72 kDa why is the actual band size different from the predicted?



    This data was developed using ab62331, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST. Exposure times:

    Lanes 1-2: 5.5 seconds

    Lane 3: 3.25 seconds

    We are unsure as to the identity of the extra band in HCT 116 and MCF7.

    The expression profile observed in HCT 116 and MCF7 are consistent with the literatures (PMID: 14739610, 24325753, 16997132).

    Negative control: HCT 116 (PMID: 14739610) and MCF7 (PMID: 24325753, 16997132).

  • Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] - BSA and Azide free (ab221924)
    Western blot - Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] - BSA and Azide free (ab221924)
    Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] (ab62331) at 1/500 dilution (unpurified) + Raw264.7 cell lysate (10ug) + LPS

    Secondary
    Goat anti-Rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 69 kDa
    Observed band size: 72 kDa why is the actual band size different from the predicted?



    This data was developed using ab62331, the same antibody clone in a different buffer formulation.

  • Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] - BSA and Azide free (ab221924)
    Anti-COX2 / Cyclooxygenase 2 antibody [EP1978Y] - BSA and Azide free (ab221924)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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