Anti-Cystathionase/CTH antibody [EPR15468] - BSA and Azide free (ab251003)
![Anti-Cystathionase/CTH antibody [EPR15468] - BSA and Azide free (ab251003)](https://www.abcam.com/ps/products/251/ab251003/Images/ab251003-408235-anti-cystathionase-cth-antibody-epr15468-bsa-and-azide-free-western-blot.jpg "Anti-Cystathionase/CTH antibody [EPR15468] - BSA and Azide free (ab251003)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15468] to Cystathionase/CTH - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Overview
-
Product name
Anti-Cystathionase/CTH antibody [EPR15468] - BSA and Azide free
See all Cystathionase/CTH primary antibodies -
Description
Rabbit monoclonal [EPR15468] to Cystathionase/CTH - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab251003 is the carrier-free version of ab189916 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251003 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Cystathionase
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR15468 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Cystathionase/CTH antibody [EPR15468] - BSA and Azide free (ab251003)
This data was developed using ab189916, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Cystathionase/CTH knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: K562 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab189916 observed at 45 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab189916 was shown to recognize Cystathionase/CTH in wild-type cells as signal was lost at the expected MW in Cystathionase/CTH knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Cystathionase/CTH knockout samples were subjected to SDS-PAGE. ab189916 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-Cystathionase/CTH antibody [EPR15468] - BSA and Azide free (ab251003)](https://www.abcam.com/ps/products/251/ab251003/Images/ab251003-405435-anti-cystathionase-cth-antibody-epr15468-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Cystathionase/CTH antibody [EPR15468] - BSA and Azide free (ab251003)Anti-Cystathionase/CTH antibody [EPR15468] - N-terminal (ab189916) at 0.01 µg/ml + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 45 kDa
Exposure time: 1 minuteThis data was developed using ab189916, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Immunoprecipitation - Anti-Cystathionase/CTH antibody [EPR15468] - BSA and Azide free (ab251003)](https://www.abcam.com/ps/products/251/ab251003/Images/ab251003-399638-anti-cystathionase-cth-antibody-epr15468-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Cystathionase/CTH antibody [EPR15468] - BSA and Azide free (ab251003)This data was developed using ab189916, the same antibody clone in a different buffer formulation.
Immunoprecipitation of Cystathionase/CTH from HeLa cell lysate using ab189916 at 1/50 or 1X PBS (negative control) (lane 1). Immunostained with ab189916 at 1/1500 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
-
![Anti-Cystathionase/CTH antibody [EPR15468] - BSA and Azide free (ab251003)](https://www.abcam.com/ps/products/251/ab251003/Images/ab251003-4-benefits-of-recombinant-antibodies.png)
Anti-Cystathionase/CTH antibody [EPR15468] - BSA and Azide free (ab251003)
