Antibodies, Proteins, Kits and Reagents for Life Science

Anti-PHGDH/Malate dehydrogenase antibody [CL0555] (ab236763)

Key features and details

Mouse monoclonal [CL0555] to PHGDH/Malate dehydrogenase
Suitable for: IHC-P, WB, ICC/IF
Reacts with: Human
Isotype: IgG1

Product name

Anti-PHGDH/Malate dehydrogenase antibody [CL0555]
See all PHGDH/Malate dehydrogenase primary antibodies
Description

Mouse monoclonal [CL0555] to PHGDH/Malate dehydrogenase
Host species

Mouse
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment corresponding to Human PHGDH/Malate dehydrogenase aa 150-350.
Database link: O43175

Run BLAST with Run BLAST with
Positive control
- WB: RT4 whole cell lysate; HeLa cytoplasmic and membrane fraction lysates. IHC-P: Human breast cancer, cerebellum, uterus, fallopian tube and prostate tissue.
General notes

This product was previously labelled as PHGDH

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

CL0555
Isotype

IgG1
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-PHGDH/Malate dehydrogenase antibody [CL0555] (ab236763)

Immunocytochemistry/Immunofluorescence analysis of MCF2 cells labelling PHGDH with ab236763 at 10 µg/ml, showing specific staining in the cytosol and the plasma membrane in green. Nuclear Probes are visualized in blue.
Western blot - Anti-PHGDH/Malate dehydrogenase antibody [CL0555] (ab236763)

Anti-PHGDH/Malate dehydrogenase antibody [CL0555] (ab236763) at 1 µg/ml + RT4 (human urinary bladder cancer cell line) whole cell lysate

Predicted band size: 57 kDa
Western blot - Anti-PHGDH/Malate dehydrogenase antibody [CL0555] (ab236763)

All lanes : Anti-PHGDH/Malate dehydrogenase antibody [CL0555] (ab236763) at 1 µg/ml

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cytoplasmic fraction
Lane 2 : HeLa membrane fraction
Lane 3 : HeLa nuclear fraction
Lane 4 : HeLa chromatin fraction
Lane 5 : HeLa cytoskeletal fraction

Predicted band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody [CL0555] (ab236763)

Paraffin-embedded human breast cancer tissue stained for PHGDH/Malate dehydrogenase using ab236763 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody [CL0555] (ab236763)

Paraffin-embedded human cerebellum tissue stained for PHGDH/Malate dehydrogenase using ab236763 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody [CL0555] (ab236763)

Paraffin-embedded human uterus tissue stained for PHGDH/Malate dehydrogenase using ab236763 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody [CL0555] (ab236763)

Paraffin-embedded human fallopian tube tissue stained for PHGDH/Malate dehydrogenase using ab236763 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody [CL0555] (ab236763)

Paraffin-embedded human prostate tissue stained for PHGDH/Malate dehydrogenase using ab236763 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody [CL0555] (ab236763)

Negative control:

Paraffin-embedded human striated muscle tissue stained for PHGDH/Malate dehydrogenase using ab236763 at 1/500 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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