Anti-PHGDH/Malate dehydrogenase antibody (ab125865)
Key features and details
- Rabbit polyclonal to PHGDH/Malate dehydrogenase
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PHGDH/Malate dehydrogenase antibody
See all PHGDH/Malate dehydrogenase primary antibodies -
Description
Rabbit polyclonal to PHGDH/Malate dehydrogenase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human PHGDH/Malate dehydrogenase aa 209-397 (internal sequence).
Database link: NP_006614 -
Positive control
- 293T, Raji, A431 and Jurkat whole cell lysates; Human breast carcinoma tissue.
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General notes
This product was previously labelled as PHGDH
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab125865 is purified by antigen-affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PHGDH/Malate dehydrogenase antibody (ab125865)All lanes : Anti-PHGDH/Malate dehydrogenase antibody (ab125865) at 1/1000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : PHGDH/Malate dehydrogenase Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 57 kDa
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Western blot - Anti-PHGDH/Malate dehydrogenase antibody (ab125865)All lanes : Anti-PHGDH/Malate dehydrogenase antibody (ab125865) at 1/10000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody (ab125865)ab125865, at 1/250, staining PHGDH/Malate dehydrogenase in formalin fixed, paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.
