Anti-PHGDH/Malate dehydrogenase antibody (ab240744)
Key features and details
- Rabbit polyclonal to PHGDH/Malate dehydrogenase
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PHGDH/Malate dehydrogenase antibody
See all PHGDH/Malate dehydrogenase primary antibodies -
Description
Rabbit polyclonal to PHGDH/Malate dehydrogenase -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Pig, Chimpanzee, Orangutan
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Immunogen
Synthetic peptide within Human PHGDH/Malate dehydrogenase aa 1-50. The exact sequence is proprietary. (NP_006614.2).
Database link: O43175 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: PHGDH/Malate dehydrogenase in HEK-293T whole cell lysate. IHC-P: Human breast carcinoma and mouse teratoma tissue.
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General notes
This product was previously labelled as PHGDH
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PHGDH/Malate dehydrogenase antibody (ab240744)All lanes : Anti-PHGDH/Malate dehydrogenase antibody (ab240744) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody (ab240744)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for PHGDH/Malate dehydrogenase using ab240744 at 1 µg/ml in immunohistochemical analysis. DAB staining.
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Immunoprecipitation - Anti-PHGDH/Malate dehydrogenase antibody (ab240744)PHGDH/Malate dehydrogenase was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab240744 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab240744 at 0.4 µg/ml.
Lane 1: ab240744 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody (ab240744)Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for PHGDH/Malate dehydrogenase using ab240744 at 1 µg/ml in immunohistochemical analysis. DAB staining.
