Anti-Histone H3 (mono methyl K18) antibody (ab272153)
Key features and details
- Rabbit polyclonal to Histone H3 (mono methyl K18)
- Suitable for: WB, ICC/IF, Dot blot
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (mono methyl K18) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (mono methyl K18) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Dot blotmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Histone H3 (mono methyl K18).
Database link: Q71DI3 -
Positive control
- WB: NIH/3T3 histone preparation; HeLa histone preparation. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3 (mono methyl K18) antibody (ab272153)Anti-Histone H3 (mono methyl K18) antibody (ab272153) at 1/500 dilution + NIH/3T3 histone preparation at 30 µg
Secondary
IRDye800™ rabbit at 1/10000 dilution
Predicted band size: 15 kDaPrimary incubation overnight at 4°C.
Block: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K18) antibody (ab272153)Immunofluorescent analysis of 0.5% PFA-fixed HeLa cells labeling Histone H3 (mono methyl K18) using ab272153 at 1/50 dilution for 1 hour at room temperature (Green). FITC secondary antibody at 1:10,000 for 45 minutes at room temperature. The nuclear counter stain is DAPI (Blue). Phalloidin staining is shown in red.
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Western blot - Anti-Histone H3 (mono methyl K18) antibody (ab272153)Anti-Histone H3 (mono methyl K18) antibody (ab272153) at 1/500 dilution + HeLa histone preparation at 30 µg
Predicted band size: 15 kDaPrimary incucbation overnight at 4°C.
Block: 5% BLOTTO overnight at 4°C.
Predicted/Observed size: ~15 kDa.
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Dot Blot - Anti-Histone H3 (mono methyl K18) antibody (ab272153)Dot Blot analysis of Histone H3 (mono methyl K18) using ab272153 at 1/1000 dilution.
Lane 1: unmodified.
Lane 2: K18Me1.
Lane 3: K18Me2.
Lane 4: K18Me3.
Lane 5: K18ac.
Load: 1, 10, and 100 picomoles of peptide.