Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Fibronectin antibody [F1] (ab32419)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [F1] to Fibronectin
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Fibronectin antibody [F1]
    See all Fibronectin primary antibodies

  • Description

    Rabbit monoclonal [F1] to Fibronectin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein within Human Fibronectin aa 1-2400. The exact sequence is proprietary.
    Database link: P02751

  • Positive control

    • WB: Human serum and plasma. SK-OV-3 and HepG2 cell lysate. IHC-P: Human breast carcinoma tissue. ICC/IF: HepG2 and human mesenchymal stem cells. Flow Cytometry: HepG2 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Fibronectin antibody [F1] (ab32419)
    Western blot - Anti-Fibronectin antibody [F1] (ab32419)
    All lanes : Anti-Fibronectin antibody [F1] (ab32419) at 1/5000 dilution (purified)

    Lane 1 : Human serum
    Lane 2 : Human plasma
    Lane 3 : SK-OV-3 (Human ovarian cancer cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 263 kDa
    Observed band size: 263 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [F1] (ab32419)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [F1] (ab32419)

    Immunohistochemical staining of paraffin embedded human breast carcinoma with purified ab32419 at a dilution of 1/250. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F1] (ab32419)
    Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F1] (ab32419)

    ICC/IF image of unpurified ab32419 stained HepG2 (Human liver hepatocellular carcinoma cell line) cells. The cells were fixed in 4% formaldehyde (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab32419 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

    Alexa Fluor® 488 (ab198933) and Alexa Fluor® 647 (ab198934) conjugated versions are available for this clone.

  • Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F1] (ab32419)
    Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F1] (ab32419) This image is courtesy of an anonymous abreview.

    ICC/IF image of unpurified ab32419 stained human mesenchymal stem cells. The cells were fixed in paraformaldehyde and then incubated in 0.1%BSA / 1% goat serum for 30 minutes, to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32419, 1/100 dilution) for 2 hours at 22°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG. DAPI was used to stain the cell nuclei (blue).

    See Abreview

  • Flow Cytometry - Anti-Fibronectin antibody [F1] (ab32419)
    Flow Cytometry - Anti-Fibronectin antibody [F1] (ab32419)

    Flow Cytometry analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Fibronectin with purified ab32419 at 1/20 dilution (10 µg/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

  • Western blot - Anti-Fibronectin antibody [F1] (ab32419)
    Western blot - Anti-Fibronectin antibody [F1] (ab32419)
    Anti-Fibronectin antibody [F1] (ab32419) at 1/1000 dilution (purified) + HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 263 kDa
    Observed band size: 263 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST

  • Anti-Fibronectin antibody [F1] (ab32419)
    Anti-Fibronectin antibody [F1] (ab32419)

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