Anti-67kDa Laminin Receptor antibody (ab99484)
Key features and details
- Rabbit polyclonal to 67kDa Laminin Receptor
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-67kDa Laminin Receptor antibody
See all 67kDa Laminin Receptor primary antibodies -
Description
Rabbit polyclonal to 67kDa Laminin Receptor -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Zebra finch
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Recombinant Human 67kDa Laminin Receptor protein (ab114294) can be used as a positive control in WB. This antibody gave a positive signal in Hu brain tissue lysate as well as the following whole cell lysates: Caco2; HCT116; LOVO; A549; HeLa.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab99484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 1 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa). Target
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Function
Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria. -
Sequence similarities
Belongs to the ribosomal protein S2P family. -
Post-translational
modificationsAcylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.
Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions. -
Cellular localization
Cell membrane. Cytoplasm. Nucleus. 67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Co-localizes with PPP1R16B in the cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 281898 Cow
- Entrez Gene: 3921 Human
- Entrez Gene: 100505031 Mouse
- Entrez Gene: 16785 Mouse
- Entrez Gene: 641351 Pig
- Entrez Gene: 29236 Rat
- Omim: 150370 Human
- SwissProt: P26452 Cow
see all -
Alternative names
- 34/67 kDa laminin receptor antibody
- 37 kDa laminin receptor precursor antibody
- 37/67 kDa laminin receptor antibody
see all
Images
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Western blot - Anti-67kDa Laminin Receptor antibody (ab99484)All lanes : Anti-67kDa Laminin Receptor antibody (ab99484) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 3 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lane 4 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-67kDa Laminin Receptor antibody (ab99484)ICC/IF image of ab99484 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99484 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol fixed (100%, 5min) HeLa, HEk293, and MCF-7 cells at 1ug/ml, and in formaldehyde fixed (4%, 10min) HeLa, HEk293, HepG2, and MCF-7 cells at 1ug/ml.
Protocols
Datasheets and documents
References (0)
ab99484 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-67kDa Laminin Receptor antibody (ab99484)All lanes : Anti-67kDa Laminin Receptor antibody (ab99484) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 3 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lane 4 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
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Immunocytochemistry/ Immunofluorescence - Anti-67kDa Laminin Receptor antibody (ab99484)
ICC/IF image of ab99484 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99484 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol fixed (100%, 5min) HeLa, HEk293, and MCF-7 cells at 1ug/ml, and in formaldehyde fixed (4%, 10min) HeLa, HEk293, HepG2, and MCF-7 cells at 1ug/ml.
