Antibodies, Proteins, Kits and Reagents for Life Science

271 382 ₸ Product size

100 µl 271 382 ₸

Order now and get it on Tuesday March 09, 2021

Anti-PRAS40 antibody [EPR6263(2)] (ab151719)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6263(2)] to PRAS40
Suitable for: WB, IHC-P, Flow Cyt
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-PRAS40 antibody [EPR6263(2)]
See all PRAS40 primary antibodies
Description

Rabbit monoclonal [EPR6263(2)] to PRAS40
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human PRAS40 aa 150-250. The exact sequence is proprietary.
Database link: Q96B36
Positive control
- 293T, SHSY5Y, HeLa and NIH 3T3 cell lysates; Human liver tissue; 293T cells.
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6263(2)
Isotype

IgG
Research areas

Western blot - Anti-PRAS40 antibody [EPR6263(2)] (ab151719)

All lanes : Anti-PRAS40 antibody [EPR6263(2)] (ab151719) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : AKT1S1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MCF-7 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 27 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab151719 observed at 27 kDa. Red - loading control, ab130007, observed at 124 kDa.

ab151719 was shown to recognize PRAS40 in wild-type HAP1 cells as signal was lost at the expected MW in AKT1S1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and AKT1S1 knockout samples were subjected to SDS-PAGE. Ab151719 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAS40 antibody [EPR6263(2)] (ab151719)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PRAS40 with ab151719 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-PRAS40 antibody [EPR6263(2)] (ab151719)

All lanes : Anti-PRAS40 antibody [EPR6263(2)] (ab151719) at 1/1000 dilution

Lane 1 : 293T cell lysate
Lane 2 : SHSY5Y cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 27 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Flow Cytometry - Anti-PRAS40 antibody [EPR6263(2)] (ab151719)

Flow cytometric analysis of permeabilized 293T cells labeling PRAS40 with ab151719 at 1/10 dilution (red), compared to a nonspecific control antibody (green).
Anti-PRAS40 antibody [EPR6263(2)] (ab151719)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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