Anti-PRAS40 (phospho S183) antibody (ab226994)
Key features and details
- Rabbit polyclonal to PRAS40 (phospho S183)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PRAS40 (phospho S183) antibody
See all PRAS40 primary antibodies -
Description
Rabbit polyclonal to PRAS40 (phospho S183) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human PRAS40 (C terminal) (phospho S183). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P00533 -
Positive control
- WB: HEK-293T, starved for 16 hours, whole cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PRAS40 (phospho S183) antibody (ab226994)All lanes : Anti-PRAS40 (phospho S183) antibody (ab226994) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), starved for 16 hours, whole cell extract
Lane 2 : HEK-293T, starved for 16 hours, and treated with 100 nM Rapamycin treated for 30 minutes whole cell extract
Lane 3 : HEK-293T, starved for 16 hours and treated with 100 nM insulin for 30 minutes, whole cell extract
Lane 4 : HEK-293T, starved for 16 hours and treated with 100 nM Rapamycin and 100 nM insulin for 30 minutes, whole cell extract
Lysates/proteins at 40 µg per lane.
Predicted band size: 27 kDa12% SDS-PAGE gel.
