Antibodies, Proteins, Kits and Reagents for Life Science

234 528 ₸ Product size

100 µl 234 528 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PRAS40 antibody [EPR6402] (ab133584)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6402] to PRAS40
Suitable for: WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-PRAS40 antibody [EPR6402]
See all PRAS40 primary antibodies
Description

Rabbit monoclonal [EPR6402] to PRAS40
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human PRAS40 aa 150-250 (internal sequence). The exact sequence is proprietary.
Positive control
- MCF7, L6, C6, RAW 264.7, PC12, NIH 3T3, 293T and HeLa whole cell lysate (ab150035)
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 1.43 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR6402
Isotype

IgG
Research areas

Western blot - Anti-PRAS40 antibody [EPR6402] (ab133584)

All lanes : Anti-PRAS40 antibody [EPR6402] (ab133584) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : AKT1S1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MCF-7 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 27 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab133584 observed at 40 kDa. Red - loading control, ab130007, observed at 125 kDa.

ab133584 was shown to specifically react with PRAS40 in wild-type HAP1 cells as signal was lost in AKT1S1 (PRAS40) knockout cells. Wild-type and AKT1S1 (PRAS40) knockout samples were subjected to SDS-PAGE. Ab133584 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-PRAS40 antibody [EPR6402] (ab133584)

All lanes : Anti-PRAS40 antibody [EPR6402] (ab133584) at 1/1000 dilution

Lane 1 : MCF7 cell lysate
Lane 2 : L6 cell lysate
Lane 3 : C6 cell lysate
Lane 4 : RAW 264.7 cell lysate
Lane 5 : PC12 cell lysate
Lane 6 : NIH 3T3 cell lysate
Lane 7 : 293T cell lysate
Lane 8 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 27 kDa
OI-RD Scanning - Anti-PRAS40 antibody [EPR6402] (ab133584)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-PRAS40 antibody [EPR6402] (ab133584)

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