Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRAS40 antibody [EPR6402] (ab133584)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6402] to PRAS40
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PRAS40 antibody [EPR6402]
    See all PRAS40 primary antibodies

  • Description

    Rabbit monoclonal [EPR6402] to PRAS40

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PRAS40 aa 150-250 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • MCF7, L6, C6, RAW 264.7, PC12, NIH 3T3, 293T and HeLa whole cell lysate (ab150035)

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PRAS40 antibody [EPR6402] (ab133584)
    Western blot - Anti-PRAS40 antibody [EPR6402] (ab133584)
    All lanes : Anti-PRAS40 antibody [EPR6402] (ab133584) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : AKT1S1 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : MCF-7 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 27 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab133584 observed at 40 kDa. Red - loading control, ab130007, observed at 125 kDa.

    ab133584 was shown to specifically react with PRAS40 in wild-type HAP1 cells as signal was lost in AKT1S1 (PRAS40) knockout cells. Wild-type and AKT1S1 (PRAS40) knockout samples were subjected to SDS-PAGE. Ab133584 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PRAS40 antibody [EPR6402] (ab133584)
    Western blot - Anti-PRAS40 antibody [EPR6402] (ab133584)
    All lanes : Anti-PRAS40 antibody [EPR6402] (ab133584) at 1/1000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : L6 cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : RAW 264.7 cell lysate
    Lane 5 : PC12 cell lysate
    Lane 6 : NIH 3T3 cell lysate
    Lane 7 : 293T cell lysate
    Lane 8 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 27 kDa

  • OI-RD Scanning - Anti-PRAS40 antibody [EPR6402] (ab133584)
    OI-RD Scanning - Anti-PRAS40 antibody [EPR6402] (ab133584)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-PRAS40 antibody [EPR6402] (ab133584)
    Anti-PRAS40 antibody [EPR6402] (ab133584)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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