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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-PRAS40 (phospho T246) antibody (ab214100)

Price and availability

264 681 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-PRAS40 (phospho T246) antibody (ab214100)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PRAS40 (phospho T246)
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PRAS40 (phospho T246) antibody
    See all PRAS40 primary antibodies
  • Description

    Rabbit polyclonal to PRAS40 (phospho T246)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human PRAS40 (phospho T246) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q96B36

  • Positive control

    • HEK293T, A431, and HeLa cell extracts; Human breast carcinoma tissue; HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.0975% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Images

  • Western blot - Anti-PRAS40 (phospho T246) antibody (ab214100)
    Western blot - Anti-PRAS40 (phospho T246) antibody (ab214100)
    All lanes : Anti-PRAS40 (phospho T246) antibody (ab214100) at 1.5 µg/ml

    Lane 1 : HEK293T cell extract
    Lane 2 : A431 cell extract
    Lane 3 : HeLa cell extract
    Lane 4 : HEK293T cell extract with non-phospho-PRAS40 peptide
    Lane 5 : HEK293T cell extract with immunizing phospho-PRAS40 (pThr246) peptide

    Secondary
    All lanes : Goat Anti-Rabbit IgG-Peroxidase

    Developed using the ECL technique.

    Predicted band size: 27 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PRAS40 (phospho T246) antibody (ab214100)
    Immunocytochemistry/ Immunofluorescence - Anti-PRAS40 (phospho T246) antibody (ab214100)

    Immunofluorescent analysis of HeLa cells labeling PRAS40 (phospho T246) with ab214100 at 10 μg/mL dilution, followed by Goat Anti-Rabbit IgG, FITC conjugate (×40 magnification). Actin staining with phalloidin rhodamine conjugate.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAS40 (phospho T246) antibody (ab214100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAS40 (phospho T246) antibody (ab214100)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling PRAS40 (phospho T246) with ab214100 at 10 μg/ml dilution followed by Goat Anti-Rabbit IgG-Biotin//ExtrAvidin®-Peroxidase.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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