Anti-PRAS40 (phospho T246) antibody (ab214100)
Key features and details
- Rabbit polyclonal to PRAS40 (phospho T246)
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PRAS40 (phospho T246) antibody
See all PRAS40 primary antibodies -
Description
Rabbit polyclonal to PRAS40 (phospho T246) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human PRAS40 (phospho T246) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: Q96B36 -
Positive control
- HEK293T, A431, and HeLa cell extracts; Human breast carcinoma tissue; HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.0975% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PRAS40 (phospho T246) antibody (ab214100) at 1.5 µg/ml
Lane 1 : HEK293T cell extract
Lane 2 : A431 cell extract
Lane 3 : HeLa cell extract
Lane 4 : HEK293T cell extract with non-phospho-PRAS40 peptide
Lane 5 : HEK293T cell extract with immunizing phospho-PRAS40 (pThr246) peptide
Secondary
All lanes : Goat Anti-Rabbit IgG-Peroxidase
Developed using the ECL technique.
Predicted band size: 27 kDa
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Immunofluorescent analysis of HeLa cells labeling PRAS40 (phospho T246) with ab214100 at 10 μg/mL dilution, followed by Goat Anti-Rabbit IgG, FITC conjugate (×40 magnification). Actin staining with phalloidin rhodamine conjugate.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling PRAS40 (phospho T246) with ab214100 at 10 μg/ml dilution followed by Goat Anti-Rabbit IgG-Biotin//ExtrAvidin®-Peroxidase.