Anti-SHANK3 antibody [N69/46] (ab193307)
![Anti-SHANK3 antibody [N69/46] (ab193307)](https://www.abcam.com/ps/products/193/ab193307/Images/ab193307-266071-anti-shank3-antibody-n69-46-western-blot.jpg "Anti-SHANK3 antibody [N69/46] (ab193307)")
Key features and details
- Mouse monoclonal [N69/46] to SHANK3
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-SHANK3 antibody [N69/46]
See all SHANK3 primary antibodies -
Description
Mouse monoclonal [N69/46] to SHANK3 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: U20S cells. Mouse cerebellum, rat cortex, rat brain membrane and human cortex lysates gave a positive result in WB.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N69/46 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-SHANK3 antibody [N69/46] (ab193307)All lanes : Anti-SHANK3 antibody [N69/46] (ab193307) at 5 µg/ml
Lane 1 : Cerebellum Mouse Tissue Lysate
Lane 2 : Rat Cortex
Lane 3 :Rat brain normal tissue lysate - membrane extract (ab29473)
Lane 4 : Human Cortex
Lane 5 : Human Striatum Neuronal cell lysate
Lane 6 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 186 kDa
Observed band size: 186 kDa
Exposure time: 4 minutes -
![Immunocytochemistry/ Immunofluorescence - Anti-SHANK3 antibody [N69/46] (ab193307)](https://www.abcam.com/ps/products/193/ab193307/Images/ab193307-247317-anti-shank3-antibody-n69-46-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SHANK3 antibody [N69/46] (ab193307)ab193307 stained U20S cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab193307 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
